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| one publication added to basket [337407] |
Molecular and cellular characterization of European sea bass CD3ε+ T lymphocytes and their modulation by microalgal feed supplementation
Picchietti, S.; Buonocore, F.; Guerra, L.; Belardinelli, M.C.; De Wolf, T.; Couto, A.; Fausto, A.M.; Saraceni, P.R.; Miccoli, A.; Scapigliati, G. (2021). Molecular and cellular characterization of European sea bass CD3ε+ T lymphocytes and their modulation by microalgal feed supplementation. Cell Tissue Res. 384(1): 149-165. https://hdl.handle.net/10.1007/s00441-020-03347-x
In: Cell and Tissue Research. Springer: Berlin; Heidelberg. ISSN 0302-766x; e-ISSN 1432-0878
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| Trefwoord |
Dicentrarchus labrax (Linnaeus, 1758) [WoRMS]
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| Author keywords |
CD3 epsilon; T lymphocytes; Sea bass; Acquired immunity; Lymphoid organs |
| Auteurs | | Top |
- Picchietti, S.
- Buonocore, F.
- Guerra, L.
- Belardinelli, M.C.
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- De Wolf, T.
- Couto, A.
- Fausto, A.M.
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- Saraceni, P.R.
- Miccoli, A.
- Scapigliati, G.
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| Abstract |
The CD3 coreceptor is a master T cell surface marker, and genes encoding CD3ζ, γδ, and ε chains have been reported in several teleost fish. Here, a complete cDNA sequence of CD3ɛ chain was identified from a sea bass (Dicentrarchus labrax L.) gill transcriptome. Its basal expression was quantified in both lymphoid and non-lymphoid organs of sea bass juveniles with real-time qPCR analysis. After either in vitro stimulation of head kidney leukocytes with the T-cell mitogen phytohaemagglutinin or in vivo stimulation with an orally administered Vibrio anguillarum vaccine, CD3ε expression levels increased in head kidney leukocytes, confirming that CD3ε T cells may play important roles in fish systemic protection against pathogens. Further, three peptides were designed on the CD3ɛ cytoplasmic tail region and employed as immunogens for antibody production in rabbit. One antiserum so obtained, named RACD3/1, immunostained a band of the expected size in a western blot of a sea bass thymocyte lysate. The distribution of CD3ε+ lymphocyte population in the lymphoid organs and mucosal tissues was addressed in healthy fish by IHC. In decreasing percentage order, CD3ε+ lymphocytes were detected by flow cytometry in thymus, peripheral blood leukocytes, gills, head kidney, gut, and spleen. Finally, a significant in vivo enhancement of CD3ε+ T intestinal lymphocytes was found in fish fed on diets in which 100% fish meal was replaced by the microalgae Nannochloropsis sp. biomass. These results indicate that CD3ε+ T cells are involved in nutritional immune responses. |
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