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| Protein responses in blue mussels (Mytilus edulis) exposed to organic pollutants: a combined CYP-antibody/proteomic approach Jonsson, H.; Schiedek, D.; Grøsvik, B.E.; Goksøyr, A. (2006). Protein responses in blue mussels (Mytilus edulis) exposed to organic pollutants: a combined CYP-antibody/proteomic approach. Aquat. Toxicol. 78(Supplement 1): S49-S56. https://dx.doi.org/10.1016/j.aquatox.2006.02.024
In: Aquatic Toxicology. Elsevier Science: Tokyo; New York; London; Amsterdam. ISSN 0166-445X; e-ISSN 1879-1514
Ook verschenen in: Pampanin, D.M.; Anderson, O.K.; Viarengo, A. (Ed.) (2006). The Stavanger Workshop: Biological Effects of Environmental Pollution (BEEP) in marine coastal ecosystem: the Stavanger mesocosm exposure studies. Aquatic Toxicology, Special Issue 78(Suppl. 1). Elsevier: The Netherlands. S1-S128 pp., meer
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Disciplines > Biology > Molecular biology > Genetics > Genomics > Functional genomics > Proteomics Enzymes > Coenzymes > Cytochromes Fauna > Aquatic organisms > Aquatic animals > Shellfish > Marine organisms > Marine molluscs Oil Phthalates Proteomics Mytilus edulis Linnaeus, 1758 [WoRMS] Marien/Kust |
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The results demonstrated that both antibodies rendered several cross-reactive bands when probed on Western blots. The most obvious cross-reaction of the CYP2 antibody was with a strongly expressed protein of size ≈57 kDa, pI 4.5–4.6, whereas the CYP4 antibody cross-reacted with a protein of size ≈55 kDa, pI 5.6. However, expression of cross-reacting proteins did not change as a result of the exposures, and resulted only in small and insignificant fluctuations in total CYP content. As a contrast, silver-stained 2DE gels showed that several microsomal proteins were affected in individuals exposed to diallylphthalate as well as crude oil, with and without a spike of alkylphenols and PAHs. Mass spectrometry based analyses of excised, trypsin-digested spots did so far not decipher the identities of the proteins affected by the exposures, nor of those cross-reacting with CYP2 and CYP4 antibodies. This study has underlined the power of the proteomic approach in environmental toxicology, although protein identification was not successful. The missing identities of the proteins cross-reacting with the CYP2- and CYP4-antibodies does not enable a clear conclusion as to whether or not these peptides actually represent CYP iso-enzymes. |
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