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| Influence of variation in methodology on the reliability of the isoelectric focusing method of fish species identification Rehbein, H.; Etienne, M.; Jerome, M.; Hattula, T.; Knudsen, L.B.; Jessen, F.; Luten, J.B.; Bouquet, W.; Mackie, I.M.; Ritchie, A.H.; Martin, R.; Mendes, R. (1995). Influence of variation in methodology on the reliability of the isoelectric focusing method of fish species identification. Food Chemistry 52(2): 193-197
In: Food Chemistry. Elsevier: London. ISSN 0308-8146; e-ISSN 1873-7072
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Chemical compounds > Organic compounds > Proteins Classification > Taxonomy > Chemotaxonomy Comparative studies Identification Products > Fishery products > Processed fishery products Oncorhynchus mykiss (Walbaum, 1792) [WoRMS] Marien/Kust; Zoet water |
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| Abstract |
In a second study, the influence of extradant (water, low ionic strength buffer, or detergent) and the position of sample application on the protein pattern was examined. Working with light muscle of rainbow trout (Oncorhynchus mykiss), it was found that the type of extractant did not influence the protein pattern. Comparison of the patterns of samples, which had been applied near the anode, in the middle, or near the cathode, revealed differences in the number and position of the protein bands under the experimental conditions applied by most laboratories. This effect was not observed with the Phast System. |
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