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Purification and characterization of vitellin from the estuarine mysid Neomysis integer (Crustacea: Mysidacea) Ghekiere, A.; Verslycke, T.; De Smet, L.; Van Beeumen, J.; Janssen, C.R. (2006). Purification and characterization of vitellin from the estuarine mysid Neomysis integer (Crustacea: Mysidacea), in: Ghekiere, A. Studie van invertebraat-specifieke effecten van endocrien-verstorende stoffen in de estuariene aasgarnaal Neomysis integer (Leach, 1814) = Study of invertebrate-specific effects of endocrine disrupting chemicals in the estuarine mysid Neomysis integer (Leach, 1814). pp. 24-35 In: Ghekiere, A. (2006). Studie van invertebraat-specifieke effecten van endocrien-verstorende stoffen in de estuariene aasgarnaal Neomysis integer (Leach, 1814) = Study of invertebrate-specific effects of endocrine disrupting chemicals in the estuarine mysid Neomysis integer (Leach, 1814). PhD Thesis. Universiteit Gent. Faculteit Bio-ingenieurswetenschappen: Gent. ISBN 90-5989-105-8. 143 pp. Is gerelateerd aan: Ghekiere, A.; Verslycke, T.; De Smet, L.; Van Beeumen, J.; Janssen, C.R. (2004). Purification and characterization of vitellin from the estuarine mysid Neomysis integer (Crustacea: Mysidacea). Comp. Biochem. Physiol. 138(4): 427-433. https://dx.doi.org/10.1016/j.cbpb.2004.05.012, meer

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Beschikbaar in	Auteurs
VLIZ: Open Marine Archive 141179 [ download pdf ]

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Trefwoorden

Secretory organs > Glands > Endocrine glands Vitellogenesis Water bodies > Coastal waters > Coastal landforms > Coastal inlets > Estuaries Neomysis integer (Leach, 1814) [WoRMS] Belgium, Zeeschelde, Galgenweel [Marine Regions] Brak water

Author keywords

crustacea; endocrine disruption; estuary; gel filtration; mysids;Neomysis integer; polyclonal antibody; vitellin

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Project	Top | Auteurs
Endocrine disruption in the Scheldt Estuary: distribution, exposure and effects

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Auteurs		Top
Ghekiere, A. Verslycke, T. De Smet, L.	Van Beeumen, J. Janssen, C.R.

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Abstract

Invertebrates account for roughly 95% of all animals, yet surprisingly, little effort has been invested to understand their value in signaling potential environmental endocrine disruption. There has been, however, much recent attention on vitellogenin induction in egg-laying invertebrates and vertebrates as indicators of exposure to estrogenic xenobiotics. Mysid shrimp (Crustacea: Mysidacea) have been put forward by several researchers and regulatory bodies (e.g., US-EPA) as suitable test organisms for the evaluation of environmental endocrine disruption. In view of developing sensitive assays to study endocrine disruption in the estuarine mysid Neomysis integer, we isolated and characterized vitellin, the major yolk protein in eggs. Vitellin was purified using gel filtration and characterized by electrophoresis using different staining procedures. Specific (as shown by Western blotting) polyclonal antibodies were produced in rabbit against the purified vitellin of N. integer. These antisera will be used to develop immunoassays to study vitellogenesis in mysids and to detect potential stimulatory or inhibitory effects of endocrine disruptors on the production of vitellin.

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