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Molecular cloning, expression, biochemical characteristics, and biomarker potential of theta class glutathione S-transferase (GST-T) from the polychaete Neanthes succinea
Rhee, J.-S.; Lee, Y.-M.; Hwang, D.-S.; Won, E.-J.; Raisuddin, S.; Shin, K.-H.; Lee, J.-S. (2007). Molecular cloning, expression, biochemical characteristics, and biomarker potential of theta class glutathione S-transferase (GST-T) from the polychaete Neanthes succinea. Aquat. Toxicol. 83(2): 104-115. http://dx.doi.org/10.1016/j.aquatox.2007.03.015
In: Aquatic Toxicology. Elsevier Science: Tokyo; New York; London; Amsterdam. ISSN 0166-445X; e-ISSN 1879-1514
|  |
| Trefwoorden |
Biological stress
Biomarkers
Chemical compounds > Organic compounds > Proteins > Metallothioneins
Chemical elements > Metals > Heavy metals
Glutathione
Nereis (Neanthes) succinea Leuckart, 1847 [WoRMS]; Polychaeta [WoRMS]
Marien/Kust |
| Author keywords |
polychaete; neanthes succinea; glutathione S-transferases; heavy metals;metallothioneins; oxidative stress; biomarker |
| Auteurs | | Top |
- Rhee, J.-S.
- Lee, Y.-M.
- Hwang, D.-S.
- Won, E.-J.
|
- Raisuddin, S.
- Shin, K.-H.
- Lee, J.-S.
|
|
| Abstract |
We cloned and sequenced the full-length cDNA of a theta class glutathione S-transferase (GST-T) from the polychaete Neanthes succinea. The open reading frame of N. succinea GST-T cDNA was 678 bp and encoded 226 amino acid residues. We generated recombinant N. succinea GST-T by expression in transformed Escherichia coli and studied the kinetic properties as well as the effects of inhibitors, pH, and temperature on N. succinea GST-T. GST-T expression was studied using real-time RT-PCR in response to exposure to the model oxidative stress-inducing agent, CuCl2. Copper induced a concentration-dependant increase in the expression of GST-T. Moreover, polychaetes collected from a heavily contaminated lake near an industrial complex showed significantly higher levels of GST-T expression. Interestingly, the site-collected polychaetes with the highest GST-T mRNA expression levels also showed the highest metallothioneins levels. These results suggest that GST-T in polychaetes may have an antioxidant role and that N. succinea GST-T expression may be a useful biomarker for exposure to environmental contaminants such as copper. Our findings provide a better understanding of the biochemical characteristics of N. succinea GST-T, and elucidate the potential role of GST-T in heavy metal-induced oxidative stress and as a biomarker for environmental contamination. |
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