Publicaties | Kaarten

nieuwe zoekopdracht

[ meld een fout in dit record ]mandje (0): toevoegen | toon Print deze pagina
High photobiological hydrogen production activity of a Nostoc sp. PCC 7422 uptake hydrogenase-deficient mutant with high nitrogenase activity
Yoshino, F.; Ikeda, H.; Masukawa, H.; Sakurai, H. (2007). High photobiological hydrogen production activity of a Nostoc sp. PCC 7422 uptake hydrogenase-deficient mutant with high nitrogenase activity. Mar. Biotechnol. 9(1): 101-112. http://dx.doi.org/10.1007/s10126-006-6035-3
In: Marine Biotechnology. Springer-Verlag: New York. ISSN 1436-2228; e-ISSN 1436-2236
Peer reviewed article  
Beschikbaar in  Auteurs 
Trefwoorden
    Chemical elements > Nonmetals > Atmospheric gases > Hydrogen
    Peptides > Proteins > Enzymes > Oxidoreductases > Nitrogenase
    Marien/Kust
Author keywords
    cyanobacteria; hydrogen; hydrogenase; nitrogenase; Nostoc;photobiological H-2 production
Auteurs  Top 
  • Yoshino, F.
  • Ikeda, H.
  • Masukawa, H.
  • Sakurai, H.
Abstract
    We describe a strategy to establish cyanobacterial strains with high levels of H2 production that involves the identification of promising wild-type strains followed by optimization of the selected strains using genetic engineering. Nostoc sp. PCC 7422 was chosen from 12 other heterocystous strains, because it has the highest nitrogenase activity. We sequenced the uptake hydrogenase (Hup) gene cluster as well as the bidirectional hydrogenase gene cluster from the strain, and constructed a mutant (ΔhupL) by insertional disruption of the hupL gene. The ΔhupL mutant produced H2 at 100 μmoles mg chlorophyll a-1 h-1, a rate three times that of the wild-type. The ΔhupL cells could accumulate H2 to about 29% (v/v) accompanied by O2 evolution in 6 days, under a starting gas phase of Ar + 5% CO2. The presence of 20% O2 in the initial gas phase inhibited H2 accumulation of the ΔhupL cells by less than 20% until day 7.
Alle informatie in het Integrated Marine Information System (IMIS) valt onder het VLIZ Privacy beleid Top | Auteurs 
IMIS is ontwikkeld en wordt gehost door het VLIZ.