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Profiling transcriptome complexity and secondary metabolite synthesis in a benthic soft coral, Sinularia polydactyla
Hoover, C.A.; Slattery, M.; Marsh, A.G. (2007). Profiling transcriptome complexity and secondary metabolite synthesis in a benthic soft coral, Sinularia polydactyla. Mar. Biotechnol. 9(2): 166-178. https://dx.doi.org/10.1007/s10126-006-6048-y
In: Marine Biotechnology. Springer-Verlag: New York. ISSN 1436-2228; e-ISSN 1436-2236
Peer reviewed article  
Beschikbaar in  Auteurs 
Trefwoorden
    Animal products > Coral
    Chemical defense
    Interspecific relationships > Predation
    Sinularia polydactyla (Ehrenberg, 1834) [WoRMS]
    Marien/Kust
Author keywords
    chemical defense; phenotypic plasticity; predation; Sinularia; softcoral transcriptome
Auteurs  Top 
  • Hoover, C.A.
  • Slattery, M.
  • Marsh, A.G.
Abstract
    Sinularia polydactyla, an abundant Indo-Pacific soft coral species, exhibits biochemical phenotypic plasticity, prompting investigations into differences in mRNA diversity and complexity in response to predation stress. Changes in transcriptome complexity of S. polydactyla cDNA libraries were measured using reannealing rate assays that employ an informatics-based analysis of kinetic profiles. This method allows for quick, high-throughput analysis of sequence complexity and has been used to compare transcriptome-level differences in other marine invertebrates. Here, S. polydactyla colonies were transplanted between two sites exhibiting high and low predation levels. Statistically significant differences between bite scar counts found on different transplant groups suggest site-specific variation in predation. Changes in mRNA pool complexity were quantified to indicate shifts in secondary metabolite concentration between treatment groups. Examining the complexity of the mRNA pool in this soft coral is one of the first steps toward understanding the mechanisms of phenotypic plasticity at a biochemical and molecular level.
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