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Development of a method for assessing the relative contribution of waterborne and dietary exposure to zinc bioaccumulation in Daphnia magna by using isotopically enriched tracers and ICP-MS detection
Balcaen, L.I.L.; De Schamphelaere, K.A.C.; Janssen, C.R.; Moens, L.; Vanhaecke, F. (2008). Development of a method for assessing the relative contribution of waterborne and dietary exposure to zinc bioaccumulation in Daphnia magna by using isotopically enriched tracers and ICP-MS detection. Anal. Bioanal. Chem. 390(2): 555-569. https://dx.doi.org/10.1007/s00216-007-1620-5
In: Analytical and Bioanalytical Chemistry. Springer: Heidelberg. ISSN 1618-2642; e-ISSN 1618-2650
Peer reviewed article  
Beschikbaar in  Auteurs 
Trefwoorden
    Biological phenomena > Accumulation > Bioaccumulation
    Chemical elements > Metals > Heavy metals > Zinc
    Chemistry
    Ecosystems
    Isotopes > Zinc isotopes
    Marine biology
    Mass spectrometry
    Mass spectrometry
    Metabolism
    Methodology
    Properties > Biological properties > Toxicity
    Solutions
    Techniques > Methodology > Analytical methods > Spectroscopy > Spectrometry > Mass spectrometry
    Toxicology > Ecotoxicology
    Water
    Water quality
    Algae [Algen]; Daphnia magna Straus, 1820 [WoRMS]
    Marien/Kust
Author keywords
    isotopic analysis; tracer study; ICP-mass spectrometry; interferences;ecotoxicology
Auteurs  Top 
  • Balcaen, L.I.L.
  • De Schamphelaere, K.A.C.
  • Janssen, C.R.
  • Moens, L.
  • Vanhaecke, F.
Abstract
    In order to study the effect of anthropogenic substances on freshwater and marine ecosystems and to develop methods to derive water-quality criteria, ecotoxicological testing is required. While toxicity assessments are traditionally based on dissolved metal concentrations, assuming that toxicity is caused by waterborne metal only, it was recently pointed out that also the dietary exposure route should be carefully considered and interpreted in regulatory assessments of zinc. In this context, the aim of this experimental study was to develop a method which allows the uptake of waterborne and dietary zinc by Daphnia magna and the interaction between both exposure routes to be studied. Therefore, the setup of a dual isotopic tracer study was required. During several days, daphnids were exposed to 67Zn and 68Zn via the dietary and the waterborne routes, respectively, and after several time intervals the daphnids were sampled and subjected to isotopic analysis by means of inductively coupled plasma mass spectrometry (ICP-MS). In order to obtain reliable and accurate results for zinc, special care was taken to prevent contamination and to deal with the spectral interferences traditionally hindering the determination of zinc. The figures of merit of both a quadrupole-based ICP-MS instrument equipped with a dynamic reaction cell, and a sector field ICP-MS unit were studied, and it was concluded that by using a sector field mass spectrometer operated at medium mass resolution all interferences could be overcome adequately. Although the set-up of the exposure experiments seems to be rather simple at first sight, it was shown in this work that several (dynamic) variables can have an influence on the results obtained and on the subsequent data interpretation. The importance of these confounding factors was examined, and on the basis of preliminary calculations it became clear that not only the isotopic composition of the daphnids has to be studied-adequate monitoring of the isotopic composition of the dissolved phase and the algae during the exposure of the daphnids is also required to accurately discriminate between uptake from water and from food.
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