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Lysozyme activity in rainbow trout, Oncorhynchus mykiss (Walbaum), stressed by handling, transport and water pollution
Möck, A.; Peters, G. (1990). Lysozyme activity in rainbow trout, Oncorhynchus mykiss (Walbaum), stressed by handling, transport and water pollution. J. Fish Biol. 37(6): 873-885. https://dx.doi.org/10.1111/j.1095-8649.1990.tb03591.x
In: Journal of Fish Biology. Fisheries Society of the British Isles: London,New York,. ISSN 0022-1112; e-ISSN 1095-8649, meer
Peer reviewed article  
Beschikbaar in  Auteurs 
Trefwoorden
    Biological stress
    Enzymatic activity
    Enzymes
    Fish culture
    Handling > Fish handling
    Pollution > Water pollution
    Stress
    Transportation
    Oncorhynchus mykiss (Walbaum, 1792) [WoRMS]
    Zoet water
Auteurs  Top 
  • Möck, A.
  • Peters, G.
Abstract
    Blood lysozyme activity was determined in rainbow trout (Salmo gairdneri) that were stressed by handling and transport, or suffered from acute ammonia poisoning. To optimize the lysoplate assay, the pH dependence of the lysozyme activity was studied. For serum and kidney lysozyme, the pH optimum was 5 multiplied by 5 to 6 multiplied by 0. Ventilation frequency and plasma glucose concentration of the fish were used as stress indicators. Less severely stressful situations, such as 30 min of handling, brought about either a stimulation or a suppression of the lysozyme activity. Strong stressors, such as transport lasting 2 h or acute water pollution, reduced the lysozyme level significantly. The decrease of lysozyme activity due to handling and transport persisted for 24 h, but the activity returned to normal within 2 weeks following confrontation with the stressor.
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