Catalogus

Zoeken

Zoeken kan via de modus 'eenvoudig zoeken' (één veld) of uitgebreid via 'geavanceerd zoeken' (meerdere velden). Zo kan je bv. zoeken op een combinatie van een auteursnaam (auteur), een jaartal (jaar) en een documenttype.

Boekenmand

Nuttige resultaten kan je aanvinken en toevoegen aan een mandje. De inhoud hiervan kan je exporteren of afdrukken (naar bv. PDF).

RSS

Op de hoogte blijven van nieuw toegevoegde publicaties binnen uw interessegebied? Dit kan door een RSS-feed (?) te maken van jouw zoekopdracht.

log in

Publicaties | Kaarten

nieuwe zoekopdracht

[ meld een fout in dit record ]

mandje (0): toevoegen | toon

---

Binding characteristics of an androgen receptor in the ovaries of coho salmon, Oncorhynchus kisutch Fitzpatrick, M.S.; Gale, W.L.; Schreck, C.B. (1994). Binding characteristics of an androgen receptor in the ovaries of coho salmon, Oncorhynchus kisutch. Gen. Comp. Endocrinol. 95: 399-408. https://dx.doi.org/10.1006/gcen.1994.1139 In: General and Comparative Endocrinology. Elsevier: New York,. ISSN 0016-6480; e-ISSN 1095-6840

---

Beschikbaar in	Auteurs
VLIZ [ aanvragen ]

---

Trefwoorden

Analysis > Biochemical analysis Biology > Physiology > Animal physiology > Fish physiology Biology > Physiology > Endocrinology Developmental stages > Juveniles Reproduction > Androgenesis Secretory organs > Glands > Endocrine glands > Animal reproductive organs > Gonads > Ovaries Sex determination Sex reversal Oncorhynchus kisutch (Walbaum, 1792) [WoRMS] Marien/Kust

---

Auteurs		Top
Fitzpatrick, M.S. Gale, W.L. Schreck, C.B.

---

Abstract

Androgen receptors were identified in the cytosol from ovaries of juvenile coho salmon, Oncorhynchus kisutch. The binding for the synthetic androgen mibolerone was specific and saturable (Kd = 0.32 plus or minus 0.02 nM; Bmax = 15.31 plus or minus 4.31 fmol/mg protein). Bound [3H]mibolerone was much higher in ovarian cytosol than in cytosolic extracts from heart, liver, and muscle. [ 3H]mibolerone specific binding was 50% lower in the plasma than in the ovarian cytosolic extracts. [ 3H]mibolerone binding was displaced most effectively by those 17 alpha -methylated synthetic androgens (mibolerone, methyltestosterone, methylandrostanolone) that can induce functional masculinization in fish. The naturally occurring androgens 11-ketotestosterone and 5 alpha -dihydrotestosterone both displaced [3H]mibolerone binding, but they were 10- to 100-fold less effective than the 17 alpha -methylated androgens. Testosterone, 11 beta -hydroxyandrostenedione, estradiol, progesterone, and 17 alpha ,20 beta -dihydroxyprogesterone were not potent competitors. [ 3H]mibolerone specific binding was reduced after preincubation with trypsin. About 25% of the binding in the cytosolic extract had DNA binding affinity under experimental conditions. The characteristics of this androgen binding site are consistent with a model of receptor-mediated steroid-induced sex inversion.

---

Alle informatie in het Integrated Marine Information System (IMIS) valt onder het VLIZ Privacy beleid

Top | Auteurs