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Production of transgenic rainbow trout (Abstract)
Penman, D.; Maclean, N. (1989). Production of transgenic rainbow trout (Abstract), in: De Pauw, N. et al. (Ed.) Aquaculture: a biotechnology in progress: volume 1. pp. 565
In: De Pauw, N. et al. (1989). Aquaculture: a biotechnology in progress: volume 1. European Aquaculture Society: Bredene. ISBN 90-71625-03-6. 1-592 pp.
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Documenttype: Congresbijdrage
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  • Penman, D.
  • Maclean, N.
Abstract
    The introduction of novel cloned genes by microinjection into eggs has produced interesting results in the last few years, particularly in mice. It seems likely that in the near future this technique will prove useful in agriculture and aquaculture, the chief limitation being the availability of potentially useful genes, once the difficulties of introduction have been overcome for each species. In our experiments, copies of a mouse metallothionein-rat growth hormone fusion gene -have been injected into rainbow trout eggs (growth hormone, somatotrophin, is a protein not a steroid, and thus should present no potential problems to the consumer since it would be digested in the gut). DNA extractions from fry revealed a low (approximately 5%) level of integration of this gene into the genome of the trout in one experiment. We have now identified transgenics at the post-fingerling stage by analysis of biopsy blood-samples from other fish, which were allowed to grow on to this stage. Some fish transgenic for the complete mouse metallothionein gene alone have also been produced. Currently we are attempting to increase the frequency of incorporation of such genes into the trout genome by varying the DNA concentration, the buffer composition, and the time and precise location of microinjection. We also hope to report on assays for circulating growth hormone in transgenic fish.
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