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Evidence for chemoautotrophic symbiosis in a Mediterranean cold seep clam (Bivalvia: Lucinidae): comparative sequence analysis of bacterial 16S rRNA, APS reductase and RubisCOgenes
Duperron, S.; Fiala-Médioni, A.; Caprais, J.-C.; Olu, K.; Sibuet, M. (2007). Evidence for chemoautotrophic symbiosis in a Mediterranean cold seep clam (Bivalvia: Lucinidae): comparative sequence analysis of bacterial 16S rRNA, APS reductase and RubisCOgenes. FEMS Microbiol. Ecol. 59(1): 64-70
In: FEMS Microbiology Ecology. Federation of European Microbiological Societies: Amsterdam. ISSN 0168-6496; e-ISSN 1574-6941
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| Trefwoorden |
Chemical compounds > Sulphur compounds > Oxides > Sulphur oxides Interspecific relationships > Symbiosis MED, Eastern Mediterranean Microorganisms > Bacteria Sediments > Chemical sediments > Sulphide deposits Lucinidae J. Fleming, 1828 [WoRMS] Mediterranean Sea - Eastern Basin [Marine Regions] Marien/Kust |
| Auteurs | | Top |
- Duperron, S.
- Fiala-Médioni, A.
- Caprais, J.-C.
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| Abstract |
Symbioses between lucinid clams (Bivalvia: Lucinidae) and autotrophic sulphide-oxidizing bacteria have mainly been studied in shallow coastal species, and information regarding deep-sea species is scarce. Here we study the symbiosis of a clam, resembling Lucinoma kazani, which was recently collected in sediment cores from new cold-seep sites in the vicinity of the Nile deep-sea fan, eastern Mediterranean, at depths ranging from 507 to 1691 m. A dominant bacterial phylotype, related to the sulphide-oxidizing symbiont of Lucinoma aequizonata, was identified in gill tissue by comparative 16S rRNA gene sequence analysis. A second phylotype, related to spirochete sequences, was identified twice in a library of 94 clones. Comparative analyses of gene sequences encoding the APS reductase α subunit and ribulose-1,5-bisphosphate carboxylase oxygenase support the hypothesis that the dominant symbiont can perform sulphide oxidation and autotrophy. Transmission electron micrographs of gills confirmed the dominance of sulphide-oxidizing bacteria, which display typical vacuoles, and δ13C values measured in gill and foot tissue further support the hypothesis for a chemoautotrophic-sourced host carbon nutrition. |
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