Zoeken
Zoeken kan via de modus 'eenvoudig zoeken' (één veld) of uitgebreid via 'geavanceerd zoeken' (meerdere velden). Zo kan je bv. zoeken op een combinatie van een auteursnaam (auteur), een jaartal (jaar) en een documenttype.
Boekenmand
Nuttige resultaten kan je aanvinken en toevoegen aan een mandje. De inhoud hiervan kan je exporteren of afdrukken (naar bv. PDF).
RSS
Op de hoogte blijven van nieuw toegevoegde publicaties binnen uw interessegebied? Dit kan door een RSS-feed (?) te maken van jouw zoekopdracht.
nieuwe zoekopdracht
Depressing effects of protein kinases A & C on the receptor- induced K+-current responses in the ganglion cells of Aplysia
Sasaki, K.; Matsumoto, G.; Takashima, K.; Fujita, R.; Kawasaki, K.; Kimura, D.K.; Sato, M. (1994). Depressing effects of protein kinases A & C on the receptor- induced K+-current responses in the ganglion cells of Aplysia. Neth. J. Zool. 44(3-4): 578-587
In: Netherlands Journal of Zoology. E.J. Brill: Leiden. ISSN 0028-2960; e-ISSN 1568-542X
|  |
| Auteurs | | Top |
- Sasaki, K.
- Matsumoto, G.
- Takashima, K.
- Fujita, R.
|
- Kawasaki, K.
- Kimura, D.K.
- Sato, M.
|
|
| Abstract |
Application of either dopamine (DA), acetylcholine (ACh), histamine (Ha), or Phe-Met-Arg-Phe-NH2 (FMRFamide) induces a K+-current response in the ganglion cells of Aplysia under voltage clamp. We have previously reported that these responses are all mediated by a pertussis toxin (PTX)-sensitive G-protein Gi or Go. Intracellular application of cAMP, an activator of protein kinase A (PKA), or extracellular application of 30 µM phorbol dibutyrate (PDBu), an activator of protein kinase C (PKC), markedly suppressed these transmitter-induced K+-current responses. The depressing effect of cAMP was reversible while that of PDBu irreversible as observed for 1 hour. Intracellular injection of okadaic acid (OA), an inhibitor phosphatases, facilitated the blocking effects of both cAMP and PDBu. The dose-response curve obtained by each transmitter-receptor system shifted downward by application of either cAMP or PDBu without affecting the affinity of the agonist to each receptor. K+-channel opening directly induced by guanosine thiotriphosphate (GTPgammaS) or by raising the temperature was not depressed y either cAMP or 100 µM PDBu. From these results, we postulated that the acting sites of both PKA and PKC might be somewhere between the receptors and G-protein, and that the phosphorylation of these sites would the functional coupling efficiency between the receptors and G-protein. |
IMIS is ontwikkeld en wordt gehost door het VLIZ.
