Zoeken
Zoeken kan via de modus 'eenvoudig zoeken' (één veld) of uitgebreid via 'geavanceerd zoeken' (meerdere velden). Zo kan je bv. zoeken op een combinatie van een auteursnaam (auteur), een jaartal (jaar) en een documenttype.
Boekenmand
Nuttige resultaten kan je aanvinken en toevoegen aan een mandje. De inhoud hiervan kan je exporteren of afdrukken (naar bv. PDF).
RSS
Op de hoogte blijven van nieuw toegevoegde publicaties binnen uw interessegebied? Dit kan door een RSS-feed (?) te maken van jouw zoekopdracht.
nieuwe zoekopdracht
Effect of dose and challenge routes of Vibrio spp. on co-infection with white spot syndrome virus in Penaeus vannamei
Phuoc, L.H.; Corteel, M.; Thanh, N.C.; Nauwynck, H.J.; Pensaert, M.B.; Alday-Sanz, V.; Van Den Broeck, W.; Sorgeloos, P.; Bossier, P. (2009). Effect of dose and challenge routes of Vibrio spp. on co-infection with white spot syndrome virus in Penaeus vannamei. Aquaculture 290(1-2): 61-68. dx.doi.org/10.1016/j.aquaculture.2009.02.004
In: Aquaculture. Elsevier: Amsterdam; London; New York; Oxford; Tokyo. ISSN 0044-8486; e-ISSN 1873-5622
|  |
| Trefwoorden |
Penaeus vannamei Boone, 1931 [WoRMS]; Vibrio Pacini, 1854 [WoRMS]
|
| Author keywords |
Co-infection; Challenge route; Dose; Shrimp; Vibrio; WSSV |
| Auteurs | | Top |
- Phuoc, L.H.
- Corteel, M.
- Thanh, N.C.
|
- Nauwynck, H.J.
- Pensaert, M.B.
- Alday-Sanz, V.
|
- Van Den Broeck, W.
- Sorgeloos, P.
- Bossier, P.
|
| Abstract |
This study was conducted to investigate the effect of dose and challenge routes of Vibrio spp. on co-infection with white spot syndrome virus (WSSV) in specific pathogen-free (SPF) Penaeus vannamei shrimp. Juvenile shrimp were first injected with WSSV at a dose of 30 SID50 shrimp- 1 (SID50 = shrimp infectious dose with 50% endpoint) and 24 h later with 103, 104, 105 or 106 CFU shrimp- 1 of V. campbellii. Controls did not die during the experiment, except the ones that received 106 CFU shrimp- 1 (35–65%). In WSSV-inoculated shrimp, the 100% cumulative mortality were reached at 144–360 h post injection (hpi). WSSV-infected shrimp died much faster when injected with at least 104 CFU of V. campbellii with the 100% cumulative mortality reached at 48–96 hpi of virus. The density of V. campbellii in haemolymph of co-infected moribund shrimp collected 6 h after V. campbellii injection was significantly higher than that in shrimp injected with V. campbellii only. There was no difference in the number of WSSV-infected cells between shrimp inoculated with WSSV only, compared to dually inoculated ones. Shrimp which were first injected with WSSV and 24 h (or 48 h) later exposed to 106, 107, or 108 CFU ml- 1 of V. campbellii by immersion did not show any accelerated mortality. When WSSV-infected shrimp were challenged with another Vibrio species, V. harveyi BB120, no accelerated mortality was noted in WSSV-infected shrimp injected with 106 CFU shrimp- 1 of V. harveyi BB120.In conclusion, it can be stated that the synergistic effect between WSSV and Vibrio is influenced by the dose, species and infection route of inoculation of the Vibrio bacteria. |
IMIS is ontwikkeld en wordt gehost door het VLIZ.
