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A DNA mini-barcoding system for authentication of processed fish products
Shokralla, S.; Hellberg, R.S.; Handy, S.M.; King, I.; Hajibabaei, M. (2015). A DNA mini-barcoding system for authentication of processed fish products. NPG Scientific Reports 5(15894): 11 pp. http://dx.doi.org/10.1038/srep15894
In: Scientific Reports (Nature Publishing Group). Nature Publishing Group: London. ISSN 2045-2322; e-ISSN 2045-2322
Peer reviewed article  
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  • Shokralla, S.
  • Hellberg, R.S.
  • Handy, S.M.
  • King, I.
  • Hajibabaei, M.
Abstract
    Species substitution is a form of seafood fraud for the purpose of economic gain. DNA barcoding utilizes species-specific DNA sequence information for specimen identification. Previous work has established the usability of short DNA sequences—mini-barcodes—for identification of specimens harboring degraded DNA. This study aims at establishing a DNA mini-barcoding system for all fish species commonly used in processed fish products in North America. Six mini-barcode primer pairs targeting short (127–314?bp) fragments of the cytochrome c oxidase I (CO1) DNA barcode region were developed by examining over 8,000 DNA barcodes from species in the U.S. Food and Drug Administration (FDA) Seafood List. The mini-barcode primer pairs were then tested against 44 processed fish products representing a range of species and product types. Of the 44 products, 41 (93.2%) could be identified at the species or genus level. The greatest mini-barcoding success rate found with an individual primer pair was 88.6% compared to 20.5% success rate achieved by the full-length DNA barcode primers. Overall, this study presents a mini-barcoding system that can be used to identify a wide range of fish species in commercial products and may be utilized in high throughput DNA sequencing for authentication of heavily processed fish products.
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