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Spliced leader RNA trans-splicing discovered in copepods
Yang, F.; Xu, D.; Zhuang, Y.; Yi, X.; Huang, Y.; Chen, H.; Lin, S.; Campbell, D.A.; Sturm, N.R.; Liu, G.; Zhang, H. (2015). Spliced leader RNA trans-splicing discovered in copepods. NPG Scientific Reports 5(17411): 10 pp. http://dx.doi.org/10.1038/srep17411
In: Scientific Reports (Nature Publishing Group). Nature Publishing Group: London. ISSN 2045-2322; e-ISSN 2045-2322
|   |
| Auteurs | | Top |
- Yang, F.
- Xu, D.
- Zhuang, Y.
- Yi, X.
|
- Huang, Y.
- Chen, H.
- Lin, S.
- Campbell, D.A.
|
- Sturm, N.R.
- Liu, G.
- Zhang, H.
|
| Abstract |
Copepods are one of the most abundant metazoans in the marine ecosystem, constituting a critical link in aquatic food webs and contributing significantly to the global carbon budget, yet molecular mechanisms of their gene expression are not well understood. Here we report the detection of spliced leader (SL) trans-splicing in calanoid copepods. We have examined nine species of wild-caught copepods from Jiaozhou Bay, China that represent the major families of the calanoids. All these species contained a common 46-nt SL (CopepodSL). We further determined the size of CopepodSL precursor RNA (slRNA; 108-158 nt) through genomic analysis and 3'-RACE technique, which was confirmed by RNA blot analysis. Structure modeling showed that the copepod slRNA folded into typical slRNA secondary structures. Using a CopepodSL-based primer set, we selectively enriched and sequenced copepod full-length cDNAs, which led to the characterization of copepod transcripts and the cataloging of the complete set of 79 eukaryotic cytoplasmic ribosomal proteins (cRPs) for a single copepod species. We uncovered the SL trans-splicing in copepod natural populations, and demonstrated that CopepodSL was a sensitive and specific tool for copepod transcriptomic studies at both the individual and population levels and that it would be useful for metatranscriptomic analysis of copepods. |
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