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Characterization of marine diatom-infecting virus promoters in the model diatom Phaeodactylum tricornutum
Kadono, T.; Miyagawa-Yamaguchi, A.; Kira, N.; Tomaru, Y.; Okami, T.; Yoshimatsu, T.; Hou, L.; Ohama, T.; Fukunaga, K.; Okauchi, M.; Yamaguchi, H.; Ohnishi, K.; Falciatore, A.; Adachi, M. (2015). Characterization of marine diatom-infecting virus promoters in the model diatom Phaeodactylum tricornutum. NPG Scientific Reports 5(18708): 13 pp. http://dx.doi.org/10.1038/srep18708
In: Scientific Reports (Nature Publishing Group). Nature Publishing Group: London. ISSN 2045-2322; e-ISSN 2045-2322
Peer reviewed article  
Beschikbaar in  Auteurs 
Trefwoorden
    Phaeodactylum tricornutum Bohlin, 1897 [WoRMS]
    Marien/Kust
Auteurs  Top 
  • Kadono, T.
  • Miyagawa-Yamaguchi, A.
  • Kira, N.
  • Tomaru, Y.
  • Okami, T.
  • Yoshimatsu, T.
  • Hou, L.
  • Ohama, T.
  • Fukunaga, K.
  • Okauchi, M.
  • Yamaguchi, H.
  • Ohnishi, K.
  • Falciatore, A.
  • Adachi, M.
Abstract
    Viruses are considered key players in phytoplankton population control in oceans. However, mechanisms that control viral gene expression in prominent microalgae such as diatoms remain largely unknown. In this study, potential promoter regions isolated from several marine diatom-infecting viruses (DIVs) were linked to the egfp reporter gene and transformed into the Pennales diatom Phaeodactylum tricornutum. We analysed their activity in cells grown under different conditions. Compared to diatom endogenous promoters, novel DIV promoter (ClP1) mediated a significantly higher degree of reporter transcription and translation. Stable expression levels were observed in transformants grown under both light and dark conditions, and high levels of expression were reported in cells in the stationary phase compared to the exponential phase of growth. Conserved motifs in the sequence of DIV promoters were also found. These results allow the identification of novel regulatory regions that drive DIV gene expression and further examinations of the mechanisms that control virus-mediated bloom control in diatoms. Moreover, the identified ClP1 promoter can serve as a novel tool for metabolic engineering of diatoms. This is the first report describing a promoter of DIVs that may be of use in basic and applied diatom research.
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