Zoeken
Zoeken kan via de modus 'eenvoudig zoeken' (één veld) of uitgebreid via 'geavanceerd zoeken' (meerdere velden). Zo kan je bv. zoeken op een combinatie van een auteursnaam (auteur), een jaartal (jaar) en een documenttype.
Boekenmand
Nuttige resultaten kan je aanvinken en toevoegen aan een mandje. De inhoud hiervan kan je exporteren of afdrukken (naar bv. PDF).
RSS
Op de hoogte blijven van nieuw toegevoegde publicaties binnen uw interessegebied? Dit kan door een RSS-feed (?) te maken van jouw zoekopdracht.
nieuwe zoekopdracht
Physicochemical properties of double-stranded RNA used to discover a reo-like virus from blue crab Callinectes sapidus
Bowers, H.A.; Messick, G.A.; Hanif, A.; Jagus, R.; Carrion, L.; Zmora, O.; Schott, E.J. (2010). Physicochemical properties of double-stranded RNA used to discover a reo-like virus from blue crab Callinectes sapidus. Dis. Aquat. Org. 93(1): 17-29. dx.doi.org/10.3354/dao02280
In: Diseases of Aquatic Organisms. Inter Research: Oldendorf/Luhe. ISSN 0177-5103; e-ISSN 1616-1580
|   |
| Trefwoord |
|
| Author keywords |
Reovirus · dsRNA · Disease · Callinectes sapidus · Aquaculture · Mortality |
| Auteurs | | Top |
- Bowers, H.A.
- Messick, G.A.
- Hanif, A.
- Jagus, R.
|
- Carrion, L.
- Zmora, O.
- Schott, E.J.
|
|
| Abstract |
Mortality among blue crab Callinectes sapidus in soft shell production facilities is typically 25% or greater. The harvest, handling, and husbandry practices of soft shell crab production have the potential to spread or exacerbate infectious crab diseases. To investigate the possible role of viruses in soft shell crab mortalities, we took advantage of the physicochemical properties of double-stranded RNA (dsRNA) to isolate a putative virus genome. Further characterization confirmed the presence of a reo-like virus that possesses 12 dsRNA genome segments. The virus was present in >50% of dead or dying soft shell crabs, but fewer than 5% of healthy hard crabs. Injection of the virus caused mortality and resulted in the appearance of viral RNA and virus inclusions in hemocytes. The genome of the virus was partially sequenced and the information used to develop a reverse transcription polymerase chain reaction (RT-PCR) assay that is able to detect the virus genome in as little as 7.5 pg of total RNA. The molecular tools developed during this study will allow us to quantify prevalence of the blue crab reo-like virus in captive (soft shell facilities, aquaculture operations) and wild populations and facilitate understanding of the role this virus has in blue crab life history. |
IMIS is ontwikkeld en wordt gehost door het VLIZ.
