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Cloning of urea transporters from the kidneys of two batoid elasmobranchs: evidence for a common elasmobranch urea transporter isoform
Janech, M.G.; Gefroh, H.A.; Cwengros, E.E.; Sulikowski, J.A.; Ploth, D.W.; Fitzgibbon, W.R. (2008). Cloning of urea transporters from the kidneys of two batoid elasmobranchs: evidence for a common elasmobranch urea transporter isoform. Mar. Biol. (Berl.) 153(6): 1173-1179. http://dx.doi.org/10.1007/s00227-007-0889-4
In: Marine Biology: International Journal on Life in Oceans and Coastal Waters. Springer: Heidelberg; Berlin. ISSN 0025-3162; e-ISSN 1432-1793
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| Auteurs | | Top |
- Janech, M.G.
- Gefroh, H.A.
- Cwengros, E.E.
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- Sulikowski, J.A.
- Ploth, D.W.
- Fitzgibbon, W.R.
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| Abstract |
One of the two phloretin-sensitive, facilitated urea transporters identified from the kidneys of the myliobatiform, euryhaline elasmobranch, Dasyatis sabina, a 379 amino acid protein ([D. sabina]strUT-2), was very similar to the 380 amino acid isoform (shUT) present in the kidney of the squaliform, dogfish shark, Squalus acanthias (a species that can be considered marginally euryhaline since it utilizes upper estuarine, as well as ocean habitats). To test the proposal that this isoform is a conserved urea transporter (UT) expressed in the kidneys of diverse elasmobranchs, UTs were cloned from the kidneys of a rajiform elasmobranch, the stenohaline skate, Leucoraja ocellata and another dasyatid stingray, the marginally euryhaline, Dasyatis say. Utilizing 5'/3' RACE, a 2,060 nt cDNA that encoded a phloretin-sensitive, 378 amino acid skate urea transporter ([L. ocellata]skUT-2) and a 1,683 nt cDNA that encoded a stingray 379 amino acid UT ([D. say]strUT-2) were obtained. These deduced UTs have a very high sequence identity with the known elasmobranch Uts. [L. ocellata]skUT-2 was 86% identical to [D. sabina]strUT-2 and 84% identical to [S. acanthias]shUT. [D. say]strUT-2 was 97% identical to the [D. sabina]strUT-2. These findings support the hypothesis that a conserved UT isoform is present in the kidneys of marine elasmobranchs, and is an important pathway for facilitated urea transport in the kidneys of marine elasmobranchs. |
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