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The use of sea urchin eggs as a model to investigate the effects of crassolide, a diterpene isolated from a soft coral
Pesando, D.; Graillet, C.; Braekman, J.-C.; Dubreuil, A.; Girard, J.-P.; Puiseux-Dao, S. (1991). The use of sea urchin eggs as a model to investigate the effects of crassolide, a diterpene isolated from a soft coral. Toxicology in vitro 5(5-6): 395-401. https://dx.doi.org/10.1016/0887-2333(91)90060-Q
In: Toxicology in vitro. ISSN 0887-2333
Peer reviewed article  
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  • Pesando, D.
  • Graillet, C.
  • Braekman, J.-C.
  • Dubreuil, A.
  • Girard, J.-P.
  • Puiseux-Dao, S.
Abstract
    Crassolide, a monocyclic diterpene isolated and purified from the soft coral Lobophytum crassum, inhibited the cell cleavage of sea urchin eggs without affecting fertilization. The effect was observed with concentrations above 2 × 10−5m in egg suspensions. Addition of crassolide between 5 and 40 min post-fertilization totally blocked the first cleavage, which in the control occurs 1 hr after fertilization. When added between 50 and 60 min post-fertilization, crassolide produced polynucleated cells in embryos. Crassolide did not affect the egg permeability to Na+ and Ca2+, but caused an increase of 0.2 units in the intracellular pH of fertilized eggs coupled with a proton efflux. Crassolide, which does not affect Ca2+ influx or permeability at the level of storage in reticular vesicles, could be used as a negative control when analysing calcium changes in short-term toxicological studies. The relationship between the pH increase and the cell cleavage needs further investigation.
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