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Ingestion of food pellets containing Escherichia coli overexpressing the heat-shock protein DnaK protects Penaeus vannamei (Boone) against Vibrio harveyi (Baumann) infection
Sinnasamy, S.; Noordin, N.M.; MacRae, T.H.; bin Abdullah, M.I.; Bossier, P.; Wahid, M.E.B.; Noriaki, A.; Sung, Y.Y. (2016). Ingestion of food pellets containing Escherichia coli overexpressing the heat-shock protein DnaK protects Penaeus vannamei (Boone) against Vibrio harveyi (Baumann) infection. J. Fish Dis. 39(5): 577-584. dx.doi.org/10.1111/jfd.12390
In: Journal of Fish Diseases. Blackwell Science: Oxford; London; Edinburgh; Boston; Melbourne. ISSN 0140-7775; e-ISSN 1365-2761
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| Trefwoorden |
Penaeus vannamei Boone, 1931 [WoRMS]; Vibrio Pacini, 1854 [WoRMS]
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| Author keywords |
DnaK; heat-shock proteins; Hsp70; P. vannamei; shrimp; Vibrio |
| Auteurs | | Top |
- Sinnasamy, S.
- Noordin, N.M.
- MacRae, T.H.
- bin Abdullah, M.I.
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- Bossier, P.
- Wahid, M.E.B.
- Noriaki, A.
- Sung, Y.Y.
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| Abstract |
Feeding aquatic animals with bacterial encapsulated heat-shock proteins (Hsps) is potentially a new method to combat vibriosis, an important disease affecting aquatic animals used in aquaculture. Food pellets comprised of shrimp and containing Escherichia coli overexpressing either DnaK-DnaJ-GrpE, the prokaryotic equivalents of Hsp70-Hsp40-Hsp20, or only DnaK were fed to juveniles of the white leg shrimp Penaeus vannamei, and protection against pathogenic Vibrio harveyi was determined. Maintaining pellets at different temperatures for varying lengths of time reduced the number of live adhering E. coli, as did contact with sea water, demonstrating that storage and immersion adversely affected bacterial survival and attachment to pellets. Feeding P. vannamei with E. coli did not compromise their survival, indicating that the bacteria were not pathogenic to shrimp. Feeding P. vannamei with pellets containing bacteria overproducing DnaK (approximately 60 cells g−1 pellets) boosted P. vannamei survival twofold against V. harveyi, suggesting that DnaK plays a role in Vibrio tolerance. Pellets containing DnaK were effective in providing protection to P. vannamei for up to 2 weeks before loss of viability and that DnaK encapsulated by these bacteria enhanced shrimp resistance against Vibrio infection. |
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