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Isolation of cyanophage CrV infecting Cylindrospermopsis raciborskii and the influence of temperature and irradiance on CrV proliferation
Steenhauer, L.M.; Wierenga, J.; Carreira, C; Limpens, R.W.A.L.; Koster, A.J.; Pollard, P.C.; Brussaard, C.P.D. (2016). Isolation of cyanophage CrV infecting Cylindrospermopsis raciborskii and the influence of temperature and irradiance on CrV proliferation . Aquat. Microb. Ecol. 78: 11-23. dx.doi.org/10.3354/ame01800
In: Aquatic Microbial Ecology. Inter-Research: Oldendorf/Luhe. ISSN 0948-3055; e-ISSN 1616-1564
Peer reviewed article  
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Trefwoord
Author keywords
    Invasive cyanobacteria; Cyanophage; Freshwater; Latent period; Burst size; Temperature; Light
Auteurs  Top 
  • Steenhauer, L.M.
  • Wierenga, J.
  • Carreira, C
  • Limpens, R.W.A.L.
  • Koster, A.J.
  • Pollard, P.C.
  • Brussaard, C.P.D.
Abstract
    ABSTRACT: A lytic virus that infects a European strain of the freshwater filamentous cyano-bacterium Cylindrospermopsis raciborskii was isolated from a lake in the Netherlands and partially characterised. With a genome size of 110 ± 15 kb, an icosahedral capsid of 65 ± 1 nm (n = 22) and a long non-contractile tail of 612 ± 31 nm (n = 15), this dsDNA cyanophage CrV appears to belong to the Siphoviridae family. CrV was highly host specific, not infecting other filamentous cyanobacteria species isolated from the same lake, nor 4 Australian strains of C. raciborskii. The latent period of this cyanophage was 20-24 h. Varying the irradiance affected cyanophage-host interactions: at low light (20 µmol quanta m-2 s-1) the latent period was 1.3 times longer compared with at mid light (90 µmol quanta m-2 s-1); burst size at mid light was 332 CrV per lysed host cell, at low light it was halved (48%) and at high light (250 µmol quanta m-2 s-1) the burst size was further reduced to only 14% of that of mid light. Temperature also affected the virus growth characteristics: the CrV latent period at high temperature (30°C) was reduced to just 11% (compared with a mid temperature of 22°C), but still the burst size increased to 541 CrV per lysed host cell; at low temperature (15°C) the latent period was prolonged 1.3-fold and the burst size was reduced to 43%. Our findings indicate that ecologically relevant environmental factors can affect the extent of viral lysis of C. raciborskii, advancing our understanding of the spread of this invasive cyanobacterium across Europe.
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