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Large scale MALDI-TOF MS based taxa identification to identify novel pigment producers in a marine bacterial culture collection
Stafsnes, M.H.; Dybwad, M.; Brunsvik, A.; Bruheim, P. (2013). Large scale MALDI-TOF MS based taxa identification to identify novel pigment producers in a marine bacterial culture collection. Antonie van Leeuwenhoek 103(3): 603-615. https://dx.doi.org/10.1007/s10482-012-9844-6
In: Antonie van Leeuwenhoek. Stichting Antonie van Leeuwenhoek: Amsterdam. ISSN 0003-6072; e-ISSN 1572-9699
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| Trefwoord |
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| Author keywords |
Marine bioprospecting, bacteria, Maldi-ToF MS dereplication, pigmentation, LC-MS |
| Auteurs | | Top |
- Stafsnes, M.H.
- Dybwad, M.
- Brunsvik, A.
- Bruheim, P.
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| Abstract |
A challenge in the rational exploitation of microbial culture collections is to avoid superfluous testing of replicas. MALDI-TOF MS has been shown to be an efficient dereplication tool as it can be used to discriminate between bacterial isolates at the species level. A bacterial culture collection of more than 10,000 heterotrophic marine bacterial isolates from sea-water surface layers of the Norwegian Trondheimsfjord and neighbouring coastal areas has been established. A sub-collection of pigmented isolates was earlier screened for novel carotenoids with UVA-Blue light absorbing properties. This was a comprehensive analytical task and it was observed that a significant number of extracts with identical pigment profile were recovered. Hence, this study was undertaken to explore the use of MALDI-TOF MS as a dereplication tool to quickly characterize the bacterial collection. Furthermore, LC-DAD-MS analysis of pigment profiles was performed to check if pigment profile diversity was maintained among isolates kept after the potential MALDI-TOF MS selection step. Four hundred isolates comprising both pigmented and non-pigmented isolates were used for this study. The resulting MALDI-TOF MS dendrogram clearly identified a diversity of different taxa and these were supported by the pigment profile clustering, thus linking the pigment production as species-specific properties. Although one exception was found, it can be concluded that MALDI-TOF MS dereplication is a promising pre-screening tool for more efficient screening of microbial culture collection containing pigments with potential novel properties. |
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