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A fast and direct liquid chromatography-mass spectrometry method to detect and quantify polyunsaturated aldehydes and polar oxylipins in diatoms
Kuhlisch, C.; Deicke, M.; Ueberschaar, N.; Wichard, T.; Pohnert, G. (2017). A fast and direct liquid chromatography-mass spectrometry method to detect and quantify polyunsaturated aldehydes and polar oxylipins in diatoms. Limnol. Oceanogr., Methods 15(1): 70-79. https://dx.doi.org/10.1002/lom3.10143
In: Limnology and Oceanography: Methods. American Society of Limnology and Oceanography: Waco, Tex.. ISSN 1541-5856; e-ISSN 1541-5856
Peer reviewed article  
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Trefwoorden
    Exploitable Scientific Result
    Marine Sciences
    Maritime Industries > Blue Biotech
    Scientific Community
    Scientific Publication
    Marien/Kust
Project Top | Auteurs 
  • Association of European marine biological laboratories
Auteurs  Top 
  • Kuhlisch, C.
  • Deicke, M.
  • Ueberschaar, N.
  • Wichard, T.
  • Pohnert, G.
Abstract
    Polyunsaturated aldehydes (PUAs) are a group of microalgal metabolites that have attracted a lot of attention due to their biological activity. Determination of PUAs has become an important routine procedure in plankton and biofilm investigations, especially those that deal with chemically mediated interactions. Here we introduce a fast and direct derivatization free method that allows quantifying PUAs in the nanomolar range, sufficient to undertake the analysis from cultures and field samples. The sample preparation requires one simple filtration step and the initiation of PUA formation by cell disruption. After centrifugation the samples are ready for measurement without any further handling. Within one chromatographic run this method additionally allows us to monitor the formation of the polar oxylipins arising from the cleavage of precursor fatty acids. The robust method is based on analyte separation and detection using ultra high performance liquid chromatography-atmospheric pressure chemical ionization mass spectrometry (UHPLC-APCI MS) and enables high throughput investigations by employing an analysis time of only 5 min. Our protocol thus provides an alternative and extension to existing PUA determinations based on gas chromatographymass spectrometry (GC-MS) with shorter run times and without any chemical derivatization. It also enables researchers with widely available LC-MS analytical platforms to monitor PUAs. Additionally, non-volatile oxylipins such as omega-oxo-acids and related compounds can be elucidated and monitored.
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