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Genome-wide comparison of ultraviolet and ethyl methanesulphonate mutagenesis methods for the brown alga Ectocarpus
Godfroy, O.; Peters, A.F.; Coelho, S.M.; Cock, J.M. (2015). Genome-wide comparison of ultraviolet and ethyl methanesulphonate mutagenesis methods for the brown alga Ectocarpus. Marine Genomics 24(1): 109-113. https://dx.doi.org/10.1016/j.margen.2015.03.007
In: Marine Genomics. Elsevier: Amsterdam. ISSN 1874-7787; e-ISSN 1876-7478
Peer reviewed article  
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Trefwoorden
    Exploitable Scientific Result
    Marine Sciences
    Maritime Industries > Blue Biotech
    Scientific Community
    Scientific Publication
    Marien/Kust
Author keywords
    Brown algae; Ectocarpus; Ethyl methanesulphonate; Mutagenesis;Ultraviolet
Project Top | Auteurs 
  • Association of European marine biological laboratories
Auteurs  Top 
  • Godfroy, O.
  • Peters, A.F.
  • Coelho, S.M.
  • Cock, J.M.
Abstract
    Ectocarpus has emerged as a model organism for the brown algae and a broad range of genetic and genomic resources are being generated for this species. The aim of the work presented here was to evaluate two mutagenesis protocols based on ultraviolet irradiation and ethyl methanesulphonate treatment using genome resequencing to measure the number, type and distribution of mutations generated by the two methods. Ultraviolet irradiation generated a greater number of genetic lesions than ethyl methanesulphonate treatment, with more than 400 mutations being detected in the genome of the mutagenised individual. This study therefore confirms that the ultraviolet mutagenesis protocol is suitable for approaches that require a high density of mutations, such as saturation mutagenesis or Targeting Induced Local Lesions in Genomes (TILLING).
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