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Preparation of amplicon libraries for metabarcoding of marine eukaryotes using Illumina MiSeq: the dual-PCR Method
Bourlat, S.J.; Haenel, Q.; Finnman, J.; Leray, M. (2016). Preparation of amplicon libraries for metabarcoding of marine eukaryotes using Illumina MiSeq: the dual-PCR Method, in: Bourlat, S.J. (Ed.) Marine genomics: methods and protocols. Methods in Molecular Biology, 1452: pp. 197-207. https://dx.doi.org/10.1007/978-1-4939-3774-5_13
In: Bourlat, S.J. (Ed.) (2016). Marine genomics: methods and protocols. Methods in Molecular Biology, 1452. Humana Press/Springer Science+Business Media, Inc: New York. ISBN 978-1-4939-3772-1. 253 pp. https://dx.doi.org/10.1007/978-1-4939-3774-5
In: Methods in Molecular Biology. Humana Press. ISSN 1064-3745
Peer reviewed article  
Beschikbaar in  Auteurs 
Author keywords
    Metabarcoding; Biodiversity; Microscopic eukaryotes; Illumina MiSeq
Auteurs  Top 
  • Bourlat, S.J.
  • Haenel, Q.
  • Finnman, J.
  • Leray, M.
Abstract
    This protocol details the preparation of multiplexed amplicon libraries for metabarcoding (amplicon-based) studies of microscopic marine eukaryotes. Metabarcoding studies, based on the amplification of a taxonomically informative marker from a collection of organisms or an environmental sample, can be performed to analyze biodiversity patterns or predator–prey interactions. For Metazoa, we use the mitochondrial cytochrome oxidase 1 (CO1) or the small ribosomal subunit (SSU) markers. Here, we describe a strategy for the preparation of multiplexed Illumina MiSeq libraries using a dual-PCR approach for the addition of index and adaptor sequences.
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