Zoeken
Zoeken kan via de modus 'eenvoudig zoeken' (één veld) of uitgebreid via 'geavanceerd zoeken' (meerdere velden). Zo kan je bv. zoeken op een combinatie van een auteursnaam (auteur), een jaartal (jaar) en een documenttype.
Boekenmand
Nuttige resultaten kan je aanvinken en toevoegen aan een mandje. De inhoud hiervan kan je exporteren of afdrukken (naar bv. PDF).
RSS
Op de hoogte blijven van nieuw toegevoegde publicaties binnen uw interessegebied? Dit kan door een RSS-feed (?) te maken van jouw zoekopdracht.
nieuwe zoekopdracht
Phloroglucinol-mediated Hsp70 production in crustaceans: protection against Vibrio parahaemolyticus in Artemia franciscana and Macrobrachium rosenbergii
Kumar, V.; Baruah, K.; Nguyen, D.V.; Smagghe, G.; Vossen, E.; Bossier, P. (2018). Phloroglucinol-mediated Hsp70 production in crustaceans: protection against Vibrio parahaemolyticus in Artemia franciscana and Macrobrachium rosenbergii. Frontiers in Immunology 9: 1091. https://dx.doi.org/10.3389/fimmu.2018.01091
In: Frontiers in Immunology. Frontiers Research Foundation: Lausanne. e-ISSN 1664-3224
Is gerelateerd aan:Kumar, V.; Baruah, K.; Nguyen, D.V.; Smagghe, G.; Vossen, E.; Bossier, P. (2020). Corrigendum: Phloroglucinol-mediated Hsp70 production in crustaceans: protection against Vibrio parahaemolyticus in Artemia franciscana and Macrobrachium rosenbergii. Frontiers in Immunology 11: 1860. https://hdl.handle.net/10.3389/fimmu.2020.01860, meer
|   |
| Trefwoorden |
Artemia Leach, 1819 [WoRMS]; Macrobrachium rosenbergii (De Man, 1879) [WoRMS]; Vibrio parahaemolyticus (Fujino, Okuno, Nakada, Aoyama, Fukai, Mukai & Ueho, 1951) Sakazaki, Iwanami & Fukumi, 1963 [WoRMS]
|
| Author keywords |
gnotobiotic Artemia; Vibrio parahaemolyticus; phloroglucinol; heat shockprotein 70; Macrobrachium rosenbergii |
| Auteurs | | Top |
- Kumar, V.
- Baruah, K.
- Nguyen, D.V.
|
- Smagghe, G.
- Vossen, E.
- Bossier, P.
|
|
| Abstract |
The halophilic aquatic bacterium, Vibrio parahaemolyticus, is an important aquatic pathogen, also capable of causing acute hepatopancreatic necrosis disease (AHPND) in shrimp resulting in significant economic losses. Therefore, there is an urgent need to develop anti-infective strategies to control AHPND. The gnotobiotic Artemia model is used to establish whether a phenolic compound phloroglucinol is effective against the AHPND strain V. parahaemolyticus MO904. We found that pretreatment with phloroglucinol, at an optimum concentration (30 µM), protects axenic brine shrimp larvae against V. parahaemolyticus infection and induced heat shock protein 70 (Hsp70) production (twofolds or more) as compared with the control. We further demonstrated that the Vibrio-protective effect of phloroglucinol was caused by its prooxidant effect and is linked to the induction of Hsp70. In addition, RNA interference confirms that phloroglucinol-induced Hsp70 mediates the survival of brine shrimp larvae against V. parahaemolyticus infection. The study was validated in xenic Artemia model and in a Macrobrachium rosenbergii system. Pretreatment of xenic brine shrimp larvae (30 µM) and Macrobrachium larvae (5 µM) with phloroglucinol increases the survival of xenic brine shrimp and Macrobrachium larvae against subsequent V. parahaemolyticus challenge. Taken together, our study provides substantial evidence that the prooxidant activity of phloroglucinol induces Hsp70 production protecting brine shrimp, A. franciscana, and freshwater shrimp, M. rosenbergii, against the AHPND V. parahaemolyticus strain MO904. Probably, phloroglucinol treatment might become part of a holistic strategy to control AHPND in shrimp. |
IMIS is ontwikkeld en wordt gehost door het VLIZ.
