Present research was undertaken to investigate the chemical composition, total phenolics assay, in vitro antioxidant, anti-inflammatory and insect antifeeding activity in chloroform extract of Limnophila indica (L.) Druce. GC-MS analysis revealed the occurrence of 4,5-Dimethyl-1,2,3,6,7,8,8a,8b-octahydrobiphenylene (18.3%) as a major compound. Total phenolic content, flavanoid content and orthodihydric phenol in the extract were 150.55±0.33 mg/g GAE, 40.72±0.69 mg/g CNE and 56.66±0.93 mg/g CLE, respectively. In-vitro antioxidant activity estimation through NO radical scavenging activity, DPPH radical scavenging activity, metal chelating activity of Fe+3 and reducing power activity of Fe+3 to Fe+2 resulted its potential result with IC50 values of 35.85±3.12, 28.52±1.32, 49.29±0.15 and 39.17±0.65 respectively. Anti-inflammatory activity determined using inhibition of protein denaturation method and extract showed significant activity (IB50=27.96±1.14). Chloroform extract of L. indica at the dose levels of 20% and 25% also exhibited significant antifeeding activity against Spilosoma oblique. |
