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Interplay and Targetome of the two conserved cyanobacterial sRNAs Yfr1 and Yfr2 in Prochlorococcus MED4
Lambrecht, S.J.; Kanesaki, Y.; Fuss, J.; Huettel, B.; Reinhardt, R.; Steglich, C. (2019). Interplay and Targetome of the two conserved cyanobacterial sRNAs Yfr1 and Yfr2 in Prochlorococcus MED4. NPG Scientific Reports 9(1): 15 pp. https://dx.doi.org/10.1038/s41598-019-49881-9
In: Scientific Reports (Nature Publishing Group). Nature Publishing Group: London. ISSN 2045-2322; e-ISSN 2045-2322
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| Trefwoord |
Prochlorococcus S.W.Chisholm, S.L.Frankel, R.Goericke, R.J.Olson, B.Palenik, J.B.Waterbury, L.West-Johnsrud & E.R.Zettler, 1992 [WoRMS]
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| Auteurs | | Top |
- Lambrecht, S.J.
- Kanesaki, Y.
- Fuss, J.
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- Huettel, B.
- Reinhardt, R.
- Steglich, C.
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| Abstract |
The sRNA Yfr1 and members of the Yfr2 sRNA family are almost universally present within cyanobacteria. The conserved motifs of these sRNAs are nearly complementary to each other, suggesting their ability to participate in crosstalk. The conserved motif of Yfr1 is shared by members of the Yfr10 sRNA family, members of which are otherwise less conserved in sequence, structure, and synteny compared to Yfr1. The different structural properties enable the discrimination of unique targets of Yfr1 and Yfr10. Unlike most studied regulatory sRNAs, Yfr1 gene expression only slightly changes under the tested stress conditions and is present at high levels at all times. In contrast, cellular levels of Yfr10 increase during the course of acclimation to darkness, and levels of Yfr2 increase when cells are shifted to high light or nitrogen limitation conditions. In this study, we investigated the targetomes of Yfr2, Yfr1, and Yfr10 in Prochlorococcus MED4, establishing CRAFD-Seq as a new method for identifying direct targets of these sRNAs that is applicable to all bacteria, including those that are not amenable to genetic modification. The results suggest that these sRNAs are integrated within a regulatory network of unprecedented complexity in the adjustment of carbon and nitrogen-related primary metabolism. |
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