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Low levels of interspecific DNA sequence variation of the mitochondrial 16S rRNA in North Atlantic redfish Sebastes (Pisces, Scorpaenidae)
Sundt, R.C.; Johansen, T. (1998). Low levels of interspecific DNA sequence variation of the mitochondrial 16S rRNA in North Atlantic redfish Sebastes (Pisces, Scorpaenidae). Sarsia 83: 449-452
In: Sarsia. University of Bergen. Universitetsforlaget: Bergen. ISSN 0036-4827; e-ISSN 1503-1128
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| Abstract |
During an attempt to develop a molecular method for identification of species and morphs of Atlantic Sebastes, extremely low levels of interspecific sequence variation in a mitochondrial large subunit (16S) ribosomal RNA were found. Nucleotide sequencing of a 480 base pair portion of the gene from representatives of six phenotypes of four species of Sebastes was executed. No variation was found among the individuals of ‘regular’ S. marinus, ‘giant’ S. marinus, ‘oceanic’ S. mentella, and ‘deep-sea’ S. mentella analyzed. Sequences from S. fasciatis and S. viviparous differed with only one base each from the marinus/mentella haplotype. The low level of genetic variation at this gene makes it unsuitable as a target for methods to identify North Atlantic Sebastes. Level of variation may indicate recent evolutionary divergence between the species, extremely low levels of interspecific sequence variation in a mitochondrial large subunit (16S) ribosomal RNA were found. Nucleotide sequencing of a 480 base pair portion of the gene from representatives of six phenotypes of four species of Sebastes was executed. No variation was found among the individuals of ‘regular’ S. marinus, ‘giant’ S. marinus, ‘oceanic’ S. mentella, and ‘deep-sea’ S. mentella analyzed. Sequences from S. fasciatis and S. viviparous differed with only one base each from the marinus/mentella haplotype. The low level of genetic variation at this gene makes it unsuitable as a target for methods to identify North Atlantic Sebastes. Level of variation may indicate recent evolutionary divergence between the species. |
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