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Rapid detection of CITES-listed shark fin species by loop-mediated isothermal amplification assay with potential for field use
But, G.W.-C.; Wu, H.-Y.; Shao, K.-T.; Shaw, P.-C. (2020). Rapid detection of CITES-listed shark fin species by loop-mediated isothermal amplification assay with potential for field use. NPG Scientific Reports 10(1): 14 pp. https://dx.doi.org/10.1038/s41598-020-61150-8
In: Scientific Reports (Nature Publishing Group). Nature Publishing Group: London. ISSN 2045-2322; e-ISSN 2045-2322
Peer reviewed article  
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  • But, G.W.-C.
  • Wu, H.-Y.
  • Shao, K.-T.
  • Shaw, P.-C.
Abstract
    Shark fin is a delicacy in many Asian countries. Overexploitation of sharks for shark fin trading has led to a drastic reduction in shark population. To monitor international trade of shark fin products and protect the endangered species from further population decline, we present rapid, user-friendly and sensitive diagnostic loop-mediated isothermal amplification (LAMP) and effective polymerase chain reaction (PCR) assays for all twelve CITES-listed shark species. Species-specific LAMP and PCR primers were designed based on cytochrome oxidase I (COI) and NADH2 regions. Our LAMP and PCR assays have been tested on 291 samples from 93 shark and related species. Target shark species could be differentiated from non-target species within three hours from DNA extraction to LAMP assay. The LAMP assay reported here is a simple and robust solution for on-site detection of CITES-listed shark species with shark fin products.
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