Zoeken
Zoeken kan via de modus 'eenvoudig zoeken' (één veld) of uitgebreid via 'geavanceerd zoeken' (meerdere velden). Zo kan je bv. zoeken op een combinatie van een auteursnaam (auteur), een jaartal (jaar) en een documenttype.
Boekenmand
Nuttige resultaten kan je aanvinken en toevoegen aan een mandje. De inhoud hiervan kan je exporteren of afdrukken (naar bv. PDF).
RSS
Op de hoogte blijven van nieuw toegevoegde publicaties binnen uw interessegebied? Dit kan door een RSS-feed (?) te maken van jouw zoekopdracht.
nieuwe zoekopdracht
The potential of genome-wide RAD sequences for resolving rapid radiations: a case study in Cactaceae
Bombonato, J.R.; do Amaral, D.T.; Silva, G.A.R.; Khan, G.; Moraes, E.M.; da Silva Andrade, S.C.; Eaton, D.A.R.; Alonso, D.P.; Ribolla, P.E.M.; Taylor, N.; Zappi, D.; Franco, F.F. (2020). The potential of genome-wide RAD sequences for resolving rapid radiations: a case study in Cactaceae. Mol. Phylogenet. Evol. 151: 106896. https://dx.doi.org/10.1016/j.ympev.2020.106896
In: Molecular Phylogenetics and Evolution. Elsevier: Orlando, FL. ISSN 1055-7903; e-ISSN 1095-9513
|  |
| Trefwoorden |
Cereus Ilmoni, 1830 [WoRMS]
Terrestrisch |
| Author keywords |
Cereus; Radiation; Missing data; Phylogenomics; ddRAD-Seq |
| Auteurs | | Top |
- Bombonato, J.R.
- do Amaral, D.T.
- Silva, G.A.R.
- Khan, G.
|
- Moraes, E.M.
- da Silva Andrade, S.C.
- Eaton, D.A.R.
- Alonso, D.P.
|
- Ribolla, P.E.M.
- Taylor, N.
- Zappi, D.
- Franco, F.F.
|
| Abstract |
The reconstruction of relationships within recently radiated groups is challenging even when massive amounts of sequencing data are available. The use of restriction site-associated DNA sequencing (RAD-Seq) to this end is promising. Here, we assessed the performance of RAD-Seq to infer the species-level phylogeny of the rapidly radiating genus Cereus (Cactaceae). To examine how the amount of genomic data affects resolution in this group, we used datasets and implemented different analyses. We sampled 52 individuals of Cereus, representing 18 of the 25 species currently recognized, plus members of the closely allied genera Cipocereus and Praecereus, and other 11 Cactaceae genera as outgroups. Three scenarios of permissiveness to missing data were carried out in iPyRAD, assembling datasets with 30% (333 loci), 45% (1440 loci), and 70% (6141 loci) of missing data. For each dataset, Maximum Likelihood (ML) trees were generated using two supermatrices, i.e., only SNPs and SNPs plus invariant sites. Accuracy and resolution were improved when the dataset with the highest number of loci was used (6141 loci), despite the high percentage of missing data included (70%). Coalescent trees estimated using SVDQuartets and ASTRAL are similar to those obtained by the ML reconstructions. Overall, we reconstruct a well-supported phylogeny of Cereus, which is resolved as monophyletic and composed of four main clades with high support in their internal relationships. Our findings also provide insights into the impact of missing data for phylogeny reconstruction using RAD loci. |
IMIS is ontwikkeld en wordt gehost door het VLIZ.
