Zoeken
Zoeken kan via de modus 'eenvoudig zoeken' (één veld) of uitgebreid via 'geavanceerd zoeken' (meerdere velden). Zo kan je bv. zoeken op een combinatie van een auteursnaam (auteur), een jaartal (jaar) en een documenttype.
Boekenmand
Nuttige resultaten kan je aanvinken en toevoegen aan een mandje. De inhoud hiervan kan je exporteren of afdrukken (naar bv. PDF).
RSS
Op de hoogte blijven van nieuw toegevoegde publicaties binnen uw interessegebied? Dit kan door een RSS-feed (?) te maken van jouw zoekopdracht.
nieuwe zoekopdracht
Delineating the key virulence factors and intraspecies divergence of Vibrio harveyi via whole-genome sequencing
Fu, S.; Ni, P.; Yang, Q.; Hu, H.; Wang, Q.; Ye, S.; Liu, Y. (2021). Delineating the key virulence factors and intraspecies divergence of Vibrio harveyi via whole-genome sequencing. Can. J. Microbiol. 67(3): 231-248. https://hdl.handle.net/10.1139/cjm-2020-0079
In: Canadian Journal of Microbiology = Journal Canadien de Microbiologie. National Research Council: Ottawa. ISSN 0008-4166; e-ISSN 1480-3275
|  |
| Trefwoorden |
Takifugu rubripes (Temminck & Schlegel, 1850) [WoRMS]; Vibrio harveyi (Johnson & Shunk, 1936) Baumann & Baumann, 1981 [WoRMS]
Marien/Kust |
| Author keywords |
Vibrio harveyi; tiger puffer; genetic divergence; type VI secretion system; virulence factors |
| Auteurs | | Top |
- Fu, S.
- Ni, P.
- Yang, Q.
- Hu, H.
|
|
|
| Abstract |
Vibrio harveyi is one of the major pathogens in aquaculture. To identify the key virulence factors affecting pathogenesis of V. harveyi towards fish, we conducted a field investigation for three representative fish farms infected with V. harveyi. Multilocus sequence typing (MLST) and whole-genome sequencing were conducted to delineate the phylogenetic relationship and genetic divergence of V. harveyi. A total of 25 V. harveyi strains were isolated from the diseased fish and groundwater and were subtyped into 12 sequence types by MLST. Five virulence genes, mshB, pilA, hutR, ureB, and ureG, were variably presented in the sequenced strains. The virulence gene profiles strongly correlated with the distinct pathogenicity of V. harveyi strains, with a strain harboring all five genes exhibiting the highest virulence towards fish. Phenotype assay confirmed that reduced virulence correlated with decreased motility and biofilm formation ability. Additionally, three types of type VI secretion system, namely T6SS1, T6SS2, and T6SS3, were identified in V. harveyi strains, which can be classified into six, four, and 12 subtypes, respectively. In conclusion, the results indicated that the virulence level of V. harveyi is mainly determined by the above virulence genes, which may play vital roles in environmental adaptation for V. harveyi. |
IMIS is ontwikkeld en wordt gehost door het VLIZ.
