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Optimisation of T-ISO biomass production rich in essential fatty acids: 2. Effect of different light regimes on the production of fatty acids
Tzovenis, I.; De Pauw, N.; Sorgeloos, P. (2003). Optimisation of T-ISO biomass production rich in essential fatty acids: 2. Effect of different light regimes on the production of fatty acids. Aquaculture 216(1-4): 223-242. dx.doi.org/10.1016/S0044-8486(02)00375-7
In: Aquaculture. Elsevier: Amsterdam; London; New York; Oxford; Tokyo. ISSN 0044-8486; e-ISSN 1873-5622
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| Trefwoorden |
Acids > Organic compounds > Organic acids > Fatty acids
Analytical techniques > Spectroscopic techniques > Absorption spectroscopy
Lighting > Light regime > Photoperiod
Marien/Kust |
| Author keywords |
T-ISO; photoperiod; fatty acids; photon flux density |
| Auteurs | | Top |
- Tzovenis, I.
- De Pauw, N.
- Sorgeloos, P.
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| Abstract |
It is well documented that culture conditions affect the fatty acid content of microalgae. We report in this study the fatty acid profiles and n - 3 HUFA productivity of T-ISO, a popular haptophyte in the aquaculture industry, cultured under three photoperiods (24:0, 16:08 and 12:12 h L:D) combined with three photon flux densities (PFD: 120, 220 and 460 µmol photon m-2 s-1), at 25 °C. Sampling took place in both the exponential and post-exponential (light-limited) phase. In general, fatty acid proportions were effected by a strong interaction of L:D× PFD resulting in metabolic changes difficult to be modelled. At the 12:12 and 24:0 h L:D the fatty acid pattern can be summarised as PUFA>SAFA>MUFA, while at 16:08 h L:D as SAFA>PUFA>MUFA reflecting a differential acclimation of the strain under light-dark cycles. At the 12:12 h L:D the PUFA content of biomass was significantly higher than at the other photocycles. PUFA content differences were located in the n - 3 fraction with the n - 6 content being rather constant. The n - 3/n - 6 and DHA/EPA ratios under 24:0 h or 12:12 h L:D were optimal according to the literature for fish and shellfish nutrition requirements. In contrast, the 16:08 h L:D regimes, especially at low PFD, produced inadequate ratios. The production of n - 3 HUFA in T-ISO is essentially influenced by the total photon flux available per day in a similar manner with growth. The capacity of the strain for storing lipid is limited under the conditions tested; consequently, the fatty acid content follows the biomass yield and productivity pattern. Hence, in the context of aquaculture a light regime of 12:12 h L:D and a PFD within the photolimitation-photoinhibition range offers advantages for the culture of T-ISO. If the high investment could be substantiated, continuous cultures under 24:0 h L:D at the same PFD range could serve as an optimisation basis using advanced photobioreactors. |
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