Zoeken
Zoeken kan via de modus 'eenvoudig zoeken' (één veld) of uitgebreid via 'geavanceerd zoeken' (meerdere velden). Zo kan je bv. zoeken op een combinatie van een auteursnaam (auteur), een jaartal (jaar) en een documenttype.
Boekenmand
Nuttige resultaten kan je aanvinken en toevoegen aan een mandje. De inhoud hiervan kan je exporteren of afdrukken (naar bv. PDF).
RSS
Op de hoogte blijven van nieuw toegevoegde publicaties binnen uw interessegebied? Dit kan door een RSS-feed (?) te maken van jouw zoekopdracht.
nieuwe zoekopdracht
A spatiotemporal characterisation of redox molecules in planarians, with a focus on the role of glutathione during regeneration
Bijnens, K.; Jaenen, V.; Wouters, A.; Leynen, N.; Pirotte, N.; Artois, T.; Smeets, K. (2021). A spatiotemporal characterisation of redox molecules in planarians, with a focus on the role of glutathione during regeneration. Biomolecules 11(5): 714. https://dx.doi.org/10.3390/biom11050714
In: Biomolecules. MDPI AG: Basel. ISSN 2218-273X; e-ISSN 2218-273X
|   |
| Author keywords |
planarians; wound healing and tissue regeneration; stem cells; redox molecules; reactive oxygen species; antioxidants |
| Auteurs | | Top |
- Bijnens, K.
- Jaenen, V.
- Wouters, A.
- Leynen, N.
|
- Pirotte, N.
- Artois, T.
- Smeets, K.
|
|
| Abstract |
A strict coordination between pro- and antioxidative molecules is needed for normal animal physiology, although their exact function and dynamics during regeneration and development remains largely unknown. Via in vivo imaging, we were able to locate and discriminate between reactive oxygen species (ROS) in real-time during different physiological stages of the highly regenerative planarian Schmidtea mediterranea. All ROS signals were strong enough to overcome the detected autofluorescence. Combined with an in situ characterisation and quantification of the transcription of several antioxidant genes, our data showed that the planarian gut and epidermis have a well-equipped redox system. Pharmacological inhibition or RNA interference of either side of the redox balance resulted in alterations in the regeneration process, characterised by decreased blastema sizes and delayed neurodevelopment, thereby affecting tails more than heads. Focusing on glutathione, a central component in the redox balance, we found that it is highly present in planarians and that a significant reduction in glutathione content led to regenerative failure with tissue lesions, characterised by underlying stem cell alterations. This exploratory study indicates that ROS and antioxidants are tightly intertwined and should be studied as a whole to fully comprehend the function of the redox balance in animal physiology. |
IMIS is ontwikkeld en wordt gehost door het VLIZ.
