Zoeken
Zoeken kan via de modus 'eenvoudig zoeken' (één veld) of uitgebreid via 'geavanceerd zoeken' (meerdere velden). Zo kan je bv. zoeken op een combinatie van een auteursnaam (auteur), een jaartal (jaar) en een documenttype.
Boekenmand
Nuttige resultaten kan je aanvinken en toevoegen aan een mandje. De inhoud hiervan kan je exporteren of afdrukken (naar bv. PDF).
RSS
Op de hoogte blijven van nieuw toegevoegde publicaties binnen uw interessegebied? Dit kan door een RSS-feed (?) te maken van jouw zoekopdracht.
nieuwe zoekopdracht
Spawning induction and embryo micromanipulation protocols in the Amphioxus Branchiostoma lanceolatum
Le Petillon, Y.; Bertrand, S.; Escriva, H. (2020). Spawning induction and embryo micromanipulation protocols in the Amphioxus Branchiostoma lanceolatum, in: Sprecher, S.G. Brain development: Methods and protocols. Methods in Molecular Biology, 2047: pp. 347-359. https://dx.doi.org/10.1007/978-1-4939-9732-9_19
In: Sprecher, S.G. (2020). Brain development: Methods and protocols. Second Edition. Methods in Molecular Biology, 2047. Humana Press: New York. e-ISBN 978-1-4939-9732-9. XIV, 527 pp. https://dx.doi.org/10.1007/978-1-4939-9732-9
In: Methods in Molecular Biology. Humana Press. ISSN 1064-3745
|  |
| Trefwoorden |
Scientific Publication
Branchiostoma Costa, 1834 [WoRMS]; Branchiostoma lanceolatum (Pallas, 1774) [WoRMS]
Marien/Kust |
| Author keywords |
EvoDevo, Spawning induction, Micromanipulation, Dissection, Graft experiment |
| Auteurs | | Top |
- Le Petillon, Y.
- Bertrand, S.
- Escriva, H.
|
|
|
| Abstract |
In the last decades, the cephalochordate amphioxus has reached a peculiar place in research laboratories as an excellent animal model to answer Evo/Devo questions. Nevertheless, mainly due to its restricted spawning season and to the small size of its embryos, only a few basic techniques in developmental biology could be used until recently. Fortunately, these last years, and thanks to the development of high-throughput techniques, new technical approaches have been possible, such as comparative transcriptomics and/or genomics. However, classic micromanipulation techniques are still difficult to apply. Here we present simple protocols for the manipulation of amphioxus embryos. First, we present the spawning induction method used with the European amphioxus species Branchiostoma lanceolatum. Second, we explain simple methods to manipulate the developing amphioxus embryo during the first steps of its development (before the hatching stage). These methods open many technical possibilities for future functional studies. Thus, we present here a simple technique to efficiently dechorionate a large number of embryos, we detail a protocol for the dissociation of cells during the first steps of the embryonic development and, finally, we describe micromanipulation approaches for tissue isolation during the gastrula stage. |
IMIS is ontwikkeld en wordt gehost door het VLIZ.
