Zoeken
Zoeken kan via de modus 'eenvoudig zoeken' (één veld) of uitgebreid via 'geavanceerd zoeken' (meerdere velden). Zo kan je bv. zoeken op een combinatie van een auteursnaam (auteur), een jaartal (jaar) en een documenttype.
Boekenmand
Nuttige resultaten kan je aanvinken en toevoegen aan een mandje. De inhoud hiervan kan je exporteren of afdrukken (naar bv. PDF).
RSS
Op de hoogte blijven van nieuw toegevoegde publicaties binnen uw interessegebied? Dit kan door een RSS-feed (?) te maken van jouw zoekopdracht.
nieuwe zoekopdracht
Single and combined potential of polystyrene microparticles and fluoranthene in the induction of DNA damage in haemocytes of Mediterranean mussel (Mytilus galloprovincialis)
Kolarevic, S.; Kracun-Kolarevic, M.; Maric, J.J.; Djordjevic, J.; Vukovic-Gacic, B.; Joksimovic, D.; Martinovic, R.; Bajt, O.; Ramšak, A. (2023). Single and combined potential of polystyrene microparticles and fluoranthene in the induction of DNA damage in haemocytes of Mediterranean mussel (Mytilus galloprovincialis). Mutagenesis 38(1): 3-12
In: Mutagenesis. United Kingdom Environmental Mutagen Society: Oxford. ISSN 0267-8357; e-ISSN 1464-3804
|   |
| Trefwoorden |
Scientific Publication
Mytilus galloprovincialis Lamarck, 1819 [WoRMS]
Marien/Kust |
| Author keywords |
microplastics, fluoranthene, polystyrene, genotoxicity, comet assay |
| Auteurs | | Top |
- Kolarevic, S.
- Kracun-Kolarevic, M.
- Maric, J.J.
|
- Djordjevic, J.
- Vukovic-Gacic, B.
- Joksimovic, D.
|
- Martinovic, R.
- Bajt, O.
- Ramšak, A.
|
| Abstract |
In this study, the possible ‘vector effect’ within the exposure of Mediterranean mussels (Mytilus galloprovincialis) to polystyrene microplastics with adsorbed fluoranthene was investigated by applying the multibiomarker approach. The major focus was placed on genotoxicological endpoints as to our knowledge there are no literature data on the genotoxicity of polystyrene microparticles alone or with adsorbed fluoranthene in the selected experimental organisms. DNA damage was assessed in haemocytes by comet assay and micronucleus test. For the assessment of neurotoxicity, acetylcholinesterase activity was measured in gills. Glutathione S-transferase was assessed in gills and hepatopancreas since these enzymes are induced for biotransformation and excretion of lipophilic compounds such as hydrocarbons. Finally, differences in physiological response within the exposure to polystyrene particles, fluoranthene, or particles with adsorbed fluoranthene were assessed by the variation of heart rate patterns studied by the noninvasive laser fibre-optic method. The uniform response of individual biomarkers within the exposure groups was not recorded. There was no clear pattern in variation of acetylcholinesterase or glutathione S-transferase activity which could be attributed to the treatment. Exposure to polystyrene increased DNA damage which was detected by the comet assay but was not confirmed by micronucleus formation. Data of genotoxicity assays indicated differential responses among the groups exposed to fluoranthene alone and fluoranthene adsorbed to polystyrene. Change in the heart rate patterns within the studied groups supports the concept of the Trojan horse effect within the exposure to polystyrene particles with adsorbed fluoranthene. |
IMIS is ontwikkeld en wordt gehost door het VLIZ.
