Zoeken
Zoeken kan via de modus 'eenvoudig zoeken' (één veld) of uitgebreid via 'geavanceerd zoeken' (meerdere velden). Zo kan je bv. zoeken op een combinatie van een auteursnaam (auteur), een jaartal (jaar) en een documenttype.
Boekenmand
Nuttige resultaten kan je aanvinken en toevoegen aan een mandje. De inhoud hiervan kan je exporteren of afdrukken (naar bv. PDF).
RSS
Op de hoogte blijven van nieuw toegevoegde publicaties binnen uw interessegebied? Dit kan door een RSS-feed (?) te maken van jouw zoekopdracht.
nieuwe zoekopdracht
Bioprospecting for novel bacterial sources of hydrolytic enzymes and antimicrobials in the Romanian littoral zone of the Black Sea
Ruginescu, R.; Lavin, P.; Iancu, L.; Menabit, S.; Purcarea, C. (2022). Bioprospecting for novel bacterial sources of hydrolytic enzymes and antimicrobials in the Romanian littoral zone of the Black Sea. Microorganisms 10(12): 2468. https://dx.doi.org/10.3390/microorganisms10122468
In: Microorganisms. MDPI: Basel. ISSN 2076-2607; e-ISSN 2076-2607
|   |
| Trefwoorden |
Bacteria [WoRMS]
Marien/Kust |
| Author keywords |
marine bacteria; seawater microbial diversity; Illumina sequencing; halophilic bacteria; extracellular hydrolases; lipase; carbohydrate-degrading enzymes; antimicrobial activity |
| Auteurs | | Top |
- Ruginescu, R.
- Lavin, P.
- Iancu, L.
|
|
|
| Abstract |
Marine microorganisms have evolved a large variety of metabolites and biochemical processes, providing great opportunities for biotechnologies. In the search for new hydrolytic enzymes and antimicrobial compounds with enhanced characteristics, the current study explored the diversity of cultured and uncultured marine bacteria in Black Sea water from two locations along the Romanian coastline. Microbial cell density in the investigated samples varied between 65 and 12.7 × 103 CFU·mL−1. The total bacterial community identified by Illumina sequencing of 16S rRNA gene comprised 185 genera belonging to 46 classes, mainly Gammaproteobacteria, Alphaproteobacteria, Flavobacteriia, and 24 phyla. The 66 bacterial strains isolated on seawater-based culture media belonged to 33 genera and showed variable growth temperatures, growth rates, and salt tolerance. A great fraction of these strains, including Pseudoalteromonas and Flavobacterium species, produced extracellular proteases, lipases, and carbohydrases, while two strains belonging to the genera Aquimarina and Streptomyces exhibited antimicrobial activity against human pathogenic bacteria. This study led to a broader view on the diversity of microbial communities in the Black Sea, and provided new marine strains with hydrolytic and antimicrobial capabilities that may be exploited in industrial and pharmaceutical applications. |
IMIS is ontwikkeld en wordt gehost door het VLIZ.
