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Optimisation and comparison of liquid and dry formulations of the biocontrol yeast Pichia anomala J121
Melin, P.; Håkansson, S.; Schnürer, J. (2006). Optimisation and comparison of liquid and dry formulations of the biocontrol yeast Pichia anomala J121. Appl. Microbiol. Biotechnol. 73(5): 1008-1016. https://dx.doi.org/10.1007/s00253-006-0552-x
In: Applied Microbiology and Biotechnology. Springer: Heidelberg; Berlin. ISSN 0175-7598; e-ISSN 1432-0614
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| Trefwoord |
Pichia E.C. Hansen, 1904 [WoRMS]
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| Auteurs | | Top |
- Melin, P.
- Håkansson, S.
- Schnürer, J.
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| Abstract |
The biocontrol yeast Pichia anomala J121 can effectively reduce mould growth on moist cereal grains during airtight storage. Practical use of microorganisms requires formulated products that meet a number of criteria. In this study we compared different formulations of P. anomala. The best way to formulate P. anomala was freeze-drying. The initial viability was as high as 80%, with trehalose previously added to the yeast. Freeze-dried products could be stored at temperatures as high as 30 degrees C for a year, with only a minor decrease in viability. Vacuum-drying also resulted in products with high storage potential, but the products were not as easily rehydrated as freeze-dried samples. Upon desiccating the cells using fluidised-bed drying or as liquid formulations, a storage temperature of 10 degrees C was required to maintain viability. Dependent on the type of formulation, harvesting of cells at different nutritional stresses affected the initial viabilities, e.g. the initial viability for fluidised-bed-dried cells was higher when the culture was fed with excess glucose, but for freeze-drying it was superior when cells were harvested after depletion of carbon. Using micro-silos we found that the biocontrol activity remained intact after drying, storage and rehydration for all formulations. |
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