Zoeken
Zoeken kan via de modus 'eenvoudig zoeken' (één veld) of uitgebreid via 'geavanceerd zoeken' (meerdere velden). Zo kan je bv. zoeken op een combinatie van een auteursnaam (auteur), een jaartal (jaar) en een documenttype.
Boekenmand
Nuttige resultaten kan je aanvinken en toevoegen aan een mandje. De inhoud hiervan kan je exporteren of afdrukken (naar bv. PDF).
RSS
Op de hoogte blijven van nieuw toegevoegde publicaties binnen uw interessegebied? Dit kan door een RSS-feed (?) te maken van jouw zoekopdracht.
| [ meld een fout in dit record ] | mandje (0): toevoegen | toon |
 |
| Comparing eDNA metabarcoding and conventional pelagic netting to inform biodiversity monitoring in deep ocean environments Cote, D.; McClenaghan, B.; Desforges, J.; Fahner, N.A.; Hajibabaei, M.; Chawarski, J.; Roul, S.; Singer, G.; Aubry, C.; Geoffroy, M. (2023). Comparing eDNA metabarcoding and conventional pelagic netting to inform biodiversity monitoring in deep ocean environments. ICES J. Mar. Sci./J. Cons. int. Explor. Mer 80(10): 2545-2562. https://dx.doi.org/10.1093/icesjms/fsad169
In: ICES Journal of Marine Science. Academic Press: London. ISSN 1054-3139; e-ISSN 1095-9289
|   |
| Beschikbaar in | Auteurs |
| |
| Trefwoord |
|
| Auteurs | Top | |
|
|
|
| Abstract |
The performance of environmental DNA (eDNA) metabarcoding has rarely been evaluated against conventional sampling methods in deep ocean mesopelagic environments. We assessed the biodiversity patterns generated with eDNA and two co-located conventional methods, oblique midwater trawls and vertical multinets, to compare regional and sample-level diversity. We then assessed the concordance of ecological patterns across water column habitats and evaluated how DNA markers and the level of sampling effort influenced the inferred community. We found eDNA metabarcoding characterized regional diversity well, detecting more taxa while identifying similar ecological patterns as conventional samples. Within sampling locations, eDNA metabarcoding rarely detected taxa across more than one replicate. While more taxa were found in eDNA than oblique midwater trawls within sample stations, fewer were found compared to vertical multinets. Our simulations show greater eDNA sampling effort would improve concordance with conventional methods. We also observed that using taxonomic data from multiple markers generated ecological patterns most similar to those observed with conventional methods. Patterns observed with Exact Sequence Variants were more stable across markers suggesting they are more powerful for detecting change. eDNA metabarcoding is a valuable tool for identifying and monitoring biological hotspots but some methodological adjustments are recommended for deep ocean environments. |
| Top | Auteurs |
