The objective of the present study was to assess the presence of 17 per- and polyfluoroalkyl substances (PFAS) in blood of two closely related top predators, the Scopoli's (Calonectris diomedea) and Cory's (C. borealis) shearwaters that breed allopatrically in Mediterranean and Atlantic basins. Among PFAS, perfluorooctanesulfononic acid (PFOS) and long chain perfluorinated carboxylic acids (PFCA) were detected in all samples, highlighting somehow the ubiquity of such pollutants. Scopoli's shearwaters from the Mediterranean showed significant higher levels in all PFAS when compared with those levels determined in the colony of Cory's shearwater from the Atlantic Ocean. Moreover, significant differences also arose within the Mediterranean colonies, with the colony of the Central Mediterranean (Zembra Island in Tunisia) having the lowest levels of all PFAS. Thus, our study suggests a marked geographical pattern regarding the presence of such contaminants, and emphasizes the necessity to study PFAS accumulation in birds inhabiting temperate regions.