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Bioencapsulation of the antibacterial drug sarafloxacin in nauplii of brine shrimp, Artemia franciscana
Dixon, B.A.; Van Poucke, S.O.; Chair, M.; Dehasque, M.; Nelis, H.J.; Sorgeloos, P.; De Leenheer, A.P. (1993). Bioencapsulation of the antibacterial drug sarafloxacin in nauplii of brine shrimp, Artemia franciscana, in: Seventh forum for applied biotechnology, PAND, Gent 30 September - October 1993, abstracts. pp. 60
In: (1993). Seventh forum for applied biotechnology, PAND, Gent 30 September - October 1993, abstracts. RUG: Gent. 150 pp.
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| Beschikbaar in | Auteurs |
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Documenttype: Congresbijdrage
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| Auteurs | | Top |
- Dixon, B.A.
- Van Poucke, S.O.
- Chair, M.
- Dehasque, M.
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- Nelis, H.J.
- Sorgeloos, P.
- De Leenheer, A.P.
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| Abstract |
Nauplii of the brine shrimp, Artemia franciscana, were enriched with sarafloxacin hydrochloride to determine if levels effective against 4 pathogenic strains of Vibrio. spp. could be accumulated in vivo. Al1 4 bacterial isolates used in the assay were isolated from diseased fish. Three were identified as Vibrio anquillarum, the fourth as Vibrio vulnificus using the API NE multitest method. Concentrations of sarafloxacin ranging from 1% to 40% were incorporated into the oil phase of Super Selco, a product used for increasing the nutritional value of Artemia. Predetermined numbers of nauplii from each concentration and controls, enriched only with Super Selco, were sampled at 2 hr intervals for 24 hrs. After rinsing to remove excess Selco, nauplii were resuspended in saline, and macerated in a stomacher. A modification of the Kirby Bauer Disk Diffusion method was used for antibiotic sensitivity testing. Sterile blank filter paper disks were inoculated with 20ul aliquots (in duplicate), and placed on a lawn of bacteria swabbed on Mueller-Hinton agar supplemented with 2% sodium chloride. Disks, provided by Abbott Laboratories, containing 5mcg of sarafloxacin served as the positive control standard. Zones of inhibition were measured following plate incubation for 24 hrs at 25°C.The results indicate that sarafloxacin is rapidly taken up by brine shrimp, and can be detected at 2hrs of enrichment by microbiological assay. The efficacy of sarafloxacin appears to be strain dependent. The concentration of sarafloxacin per nauplli following a 10% enrichment concentration was determined as 0.0026ug by a microbiological antibiotic assay method using 0.05% Escherichia co1i and dilution standards of sarafloxacin. |
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