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Reproduction-associated immunoreactive peptides in the nervous systems of prosobranch gastropods
Ram, J.L.; Gallardo, C.S.; Ram, M.L.; Croll, R.P. (1998). Reproduction-associated immunoreactive peptides in the nervous systems of prosobranch gastropods. Biol. Bull. 195(3): 308-318
In: The Biological Bulletin. Marine Biological Laboratory: Lancaster. ISSN 0006-3185; e-ISSN 1939-8697
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| Auteurs | | Top |
- Ram, J.L.
- Gallardo, C.S.
- Ram, M.L.
- Croll, R.P.
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| Abstract |
Antibodies against reproductive peptides of Aplysia and Lymnaea were used to localize homologous immunoreactive peptides in the nervous systems of three prosobranch species: Busycon canaliculatum, Concholepas concholepas, and Tegula atra. Positive control experiments in L. stagnalis demonstrated the broad species range of the anti-egg-laying hormone (anti-ELH) antibody used in this study, and showed binding of anti-alpha-caudodorsal-cell peptide (anti-alpha-CDCP) to the same cells in cerebral and buccal ganglia. Dot immunoassays with synthetic ELH confirmed the reactivity and sensitivity (< 0.1 microgram) of the anti-ELH antibody. Experiments with preadsorbed antibody or no primary antibody confirmed its specificity. In B. canaliculatum, clusters of more than 300 neuronal cell bodies immunoreactive to both anti-ELH and anti-alpha-CDCP were observed along the medial margins of left and right cerebral ganglia. Anti-alpha-CDCP reacted with additional small populations of cerebral ganglion neurons not stained by anti-ELH. Anti-ELH and anti-alpha-CDCP also reacted with overlapping but different small populations of neurons in buccal ganglia. In C. concholepas and T. atra, ELH-like immunoreactivity was found in cerebral ganglia, and in T. atra in fibers in the cerebral ganglia and cerebral-pedal connectives. Thus, cerebral ganglia are the major locus of the ELH-like immunoreactivity in prosobranchs. |
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