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Genome size of Adriatic seagrasses
Koce, J.D.; Vilhar, B.; Bohanec, B.; Dermastia, M. (2003). Genome size of Adriatic seagrasses. Aquat. Bot. 77(1): 17-25. http://dx.doi.org/10.1016/s0304-3770(03)00072-x
In: Aquatic Botany. Elsevier Science: Tokyo; Oxford; New York; London; Amsterdam. ISSN 0304-3770; e-ISSN 1879-1522
Peer reviewed article  
Beschikbaar in  Auteurs 
Trefwoorden
    Acids > Organic compounds > Organic acids > Nucleic acids > DNA
    Flora > Weeds > Marine organisms > Seaweeds > Sea grass
    Genomes
    MED, Adriatic
    Alismatidae; Cymodocea nodosa (Ucria) Ascherson, 1870 [WoRMS]; Pisum sativum; Posidonia oceanica (Linnaeus) Delile, 1813 [WoRMS]; Zostera marina Linnaeus, 1753 [WoRMS]; Zostera noltei Hornemann, 1832 [WoRMS]
    Adriatic Sea [Marine Regions]
    Marien/Kust
Author keywords
    seagrasses; Alismatidae; genome size; C-value; DNA image cytometry
Auteurs  Top 
  • Koce, J.D.
  • Vilhar, B.
  • Bohanec, B.
  • Dermastia, M.
Abstract
    Genome size (C-value) was measured in four species of Adriatic seagrasses with interphase-peak DNA image cytometry. The estimated 2C-value was 1.5 pg DNA for Zostera noltii (2n=12), 1.2 pg for Zostera marina (2n=12), 1.1 pg for Cymodocea nodosa (2n=28) and 6.2 pg for Posidonia oceanica, using Pisum sativum (2C-value=8.84 pg) as the calibration standard. Seagrass leaves were fixed in 4% buffered formaldehyde to mitigate stoichiometric error due to tannins and post-fixed in 3:1 methanol:acetic acid (MAA). DNA was stained with the Feulgen reaction after hydrolysis in 5 M HCl for 90 min at 20 °C. Comparison of genome size of seagrasses with the data for other species of Alismatidae indicated that the ancestral genome of Alismatidae was relatively small.
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