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Preliminary study on linkage mapping based on microsatellite DNA and AFLP markers using homozygous clonal fish in ayu (Plecoglossus altivelis)
Watanabe, T.; Fujita, H.; Yamasaki, K.; Seki, S.; Taniguchi, N. (2004). Preliminary study on linkage mapping based on microsatellite DNA and AFLP markers using homozygous clonal fish in ayu (Plecoglossus altivelis). Mar. Biotechnol. 6(4): 327-334. http://dx.doi.org/10.1007/s10126-003-0026-4
In: Marine Biotechnology. Springer-Verlag: New York. ISSN 1436-2228; e-ISSN 1436-2236
Peer reviewed article  
Beschikbaar in  Auteurs 
Trefwoorden
    Acids > Organic compounds > Organic acids > Nucleic acids > DNA
    Aflp
    Fishes > Osteichthyes > Salmoniformes > Plecoglossidae > Plecoglossus > Freshwater fishes > Ayu
    Japan
    Plecoglossus altivelis (Temminck & Schlegel, 1846) [WoRMS]
    Japan [Marine Regions]
    Marien/Kust
Author keywords
    AFLPs; ayu (Plecoglossus altivelis); linkage map; microsatellite DNA
Auteurs  Top 
  • Watanabe, T.
  • Fujita, H.
  • Yamasaki, K.
  • Seki, S.
  • Taniguchi, N.
Abstract
    A mapping referential family (F-1) of ayu was produced by crossing a normal diploid male with a homozygous clonal female. A genetic linkage map was constructed using 191 amplified fragment length polymorphism (AFLP) and 4 microsatellite DNA markers. A total of 178 loci were mapped in 36 linkage groups comprising 1659.6 cM, which includes approximately 77.3% to 81.8% of the total genome. As the markers were randomly distributed over the genome, they showed high efficiency for the construction of a wide linkage map.
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