Zoeken
Zoeken kan via de modus 'eenvoudig zoeken' (één veld) of uitgebreid via 'geavanceerd zoeken' (meerdere velden). Zo kan je bv. zoeken op een combinatie van een auteursnaam (auteur), een jaartal (jaar) en een documenttype.
Boekenmand
Nuttige resultaten kan je aanvinken en toevoegen aan een mandje. De inhoud hiervan kan je exporteren of afdrukken (naar bv. PDF).
RSS
Op de hoogte blijven van nieuw toegevoegde publicaties binnen uw interessegebied? Dit kan door een RSS-feed (?) te maken van jouw zoekopdracht.
nieuwe zoekopdracht
Tissue-specific accumulation of cadmium in subcellular compartments of eastern oysters Crassostrea virginica Gmelin (Bivalvia: Ostreidae)
Sokolova, I.M.; Ringwood, A.H.; Johnson, C. (2005). Tissue-specific accumulation of cadmium in subcellular compartments of eastern oysters Crassostrea virginica Gmelin (Bivalvia: Ostreidae). Aquat. Toxicol. 74(3): 218-228. https://dx.doi.org/10.1016/j.aquatox.2005.05.012
In: Aquatic Toxicology. Elsevier Science: Tokyo; New York; London; Amsterdam. ISSN 0166-445X; e-ISSN 1879-1514
|  |
| Trefwoorden |
Accumulation
Cell constituents > Cell organelles > Lysosomes
Cell constituents > Cell organelles > Mitochondria
Chemical elements > Metals > Heavy metals > Cadmium
Crassostrea virginica (Gmelin, 1791) [WoRMS]
Marien/Kust |
| Author keywords |
cadmium; accumulation; subcellular distribution; mitochondria;lysosomes; bivalve mollusks |
| Auteurs | | Top |
- Sokolova, I.M.
- Ringwood, A.H.
- Johnson, C.
|
|
|
| Abstract |
Cadmium distribution was studied in different subcellular fractions of gill and hepatopancreas tissues of eastern oysters Crassostrea virginica. Oysters were exposed for up to 21 days to low sublethal Cd concentrations (25 μg L−1). Gill and hepatopancreas tissues were sampled and divided into organelle fractions and cytosol by differential centrifugation. Organelle content of different fractions was verified by activities of marker enzymes, citrate synthase and acid phosphatase for mitochondria and lysosomes, respectively. In both tissue types, there was a significant accumulation of cadmium in cytosol reaching 230–350 ng mg−1 protein. Among organelles, mitochondria were the main target for Cd bioaccumulation in gills (250–300 ng mg−1 protein), whereas in hepatopancreas tissues, the highest cadmium accumulation occurred in lysosomes (90–94 ng mg−1 protein). Although 75–83% of total cadmium burden was associated with the cytosol reflecting high volume fraction of this compartment, Cd concentrations in organelle fractions reached levels that could cause dysfunction of mitochondria and lysosomes. Organ- and organelle-specific patterns of cadmium bioaccumulation support our previous in vivo studies, which showed adverse effects of cadmium exposures on mitochondrial oxidation in gills and on the lysosomal system of hepatopancreas. This may have important implications for the development of biomarkers of effect for heavy metals and for understanding the mechanisms of toxic effects of metals. |
IMIS is ontwikkeld en wordt gehost door het VLIZ.
