Catalogus

Zoeken

Zoeken kan via de modus 'eenvoudig zoeken' (één veld) of uitgebreid via 'geavanceerd zoeken' (meerdere velden). Zo kan je bv. zoeken op een combinatie van een auteursnaam (auteur), een jaartal (jaar) en een documenttype.

Boekenmand

Nuttige resultaten kan je aanvinken en toevoegen aan een mandje. De inhoud hiervan kan je exporteren of afdrukken (naar bv. PDF).

RSS

Op de hoogte blijven van nieuw toegevoegde publicaties binnen uw interessegebied? Dit kan door een RSS-feed (?) te maken van jouw zoekopdracht.

log in

Publicaties | Kaarten

nieuwe zoekopdracht

[ meld een fout in dit record ]

mandje (0): toevoegen | toon

---

Immunofluorescent detection of 8-oxo-dG and PAH bulky adducts in fish liver and mussel digestive gland Machella, N.; Regoli, F.; Santella, R.M. (2005). Immunofluorescent detection of 8-oxo-dG and PAH bulky adducts in fish liver and mussel digestive gland. Aquat. Toxicol. 71(4): 335-343. https://dx.doi.org/10.1016/j.aquatox.2004.12.002 In: Aquatic Toxicology. Elsevier Science: Tokyo; New York; London; Amsterdam. ISSN 0166-445X; e-ISSN 1879-1514

---

Beschikbaar in	Auteurs
VLIZ: Non-open access 352508 [ aanvragen ]

---

Trefwoorden

Aquatic organisms > Freshwater organisms > Fish > Freshwater fish Eels Eukaryotes > Animals > Invertebrates > Mollusca > Bivalvia > Shellfish > Mussels Fauna > Aquatic organisms > Aquatic animals > Shellfish > Marine organisms > Marine molluscs Immunofluorescence MED, Italy, Marches, Ancona MED, Italy, Tuscany, Orbetello Lagoon Mussels Organisms > Aquatic organisms > Animals > Aquatic animals > Marine animals > Fishes > Aquatic animals > Marine fishes > Osteichthyes > Eels Anguilla anguilla (Linnaeus, 1758) [WoRMS]; Mytilus galloprovincialis Lamarck, 1819 [WoRMS] Orbetello Lagoon [Marine Regions] Marien/Kust; Zoet water

Author keywords

7,8-dihydro-8-oxodeoxyguanosine; immunofluorescence staining; monoclonalantibody 1F7; PAH-DNA adducts; mussels; eels

---

Auteurs		Top
Machella, N. Regoli, F. Santella, R.M.

---

Abstract

Development of methodologies to detect DNA damage induced by pollutants is of increasing concern in marine ecotoxicology. We previously described an immunoperoxidase method for revealing 7,8-dihydro-8-oxodeoxyguanosine (8-oxo-dG) in marine organisms. In this work, the approach was extended to immunofluorescence detection and to the use of another antibody for polycyclic aromatic hydrocarbon (PAH)-DNA adducts. Specimens of European eel (Anguilla anguilla) and Mediterranean mussel (Mytilus galloprovincialis) were exposed to benzo(a)pyrene (B[a]P), as model chemical carcinogen to induce both oxidized bases and B[a]P diol-epoxide-deoxyguanosine adducts. Cryostat sections of liver and digestive gland from both species were immunostained, and DNA damage was semiquantitatively evaluated by an image analysis system. Compared to untreated organisms, B[a]P-exposed organisms exhibited increased levels of oxidative DNA damage; in eels, which rapidly metabolize PAHs, the occurrence of B[a]P-DNA adducts was also detected. The immunofluorescent assay maintained all the advantages previously reported for the immunoperoxidase protocol. Both methods were tested on the same eel specimens and the immunofluorescence method showed a greater extent of relative DNA damage and a higher sensitivity. Although field validation is being carried out, our results indicate the utility of antibodies to rapidly detect DNA alterations in aquatic organisms, and to investigate the risk associated with genotoxins in marine environment.

---

Alle informatie in het Integrated Marine Information System (IMIS) valt onder het VLIZ Privacy beleid

Top | Auteurs