Over het archief
Het OWA, het open archief van het Waterbouwkundig Laboratorium heeft tot doel alle vrij toegankelijke onderzoeksresultaten van dit instituut in digitale vorm aan te bieden. Op die manier wil het de zichtbaarheid, verspreiding en gebruik van deze onderzoeksresultaten, alsook de wetenschappelijke communicatie maximaal bevorderen.
Dit archief wordt uitgebouwd en beheerd volgens de principes van de Open Access Movement, en het daaruit ontstane Open Archives Initiative.
Basisinformatie over ‘Open Access to scholarly information'.
Validation and interpretation of CALUX as a tool for the estimation of dioxin-like activity in marine biological matrixes
Windal, I.; Van Wouwe, N.; Eppe, G.; Xhrouet, C.; Debacker, V.; Baeyens, W.F.J.; De Pauw, E.; Goeyens, L. (2005). Validation and interpretation of CALUX as a tool for the estimation of dioxin-like activity in marine biological matrixes. Environ. Sci. Technol. 39(6): 1741-1748. dx.doi.org/10.1021/es049182d
In: Environmental Science and Technology. American Chemical Society: Easton. ISSN 0013-936X; e-ISSN 1520-5851, meer
|  |
| Auteurs | | Top |
- Windal, I.
- Van Wouwe, N.
- Eppe, G.
- Xhrouet, C.
|
- Debacker, V., meer
- Baeyens, W.F.J., meer
- De Pauw, E.
- Goeyens, L., meer
|
|
| Abstract |
Among the different analytical tools proposed as an alternative to the very expensive gas chromatography high-resolution mass spectrometry (GC-HRMS) analyses of polychlorodibenzo-p-dioxin and polychlorodibenzofurans, Chemically Activated LUciferase gene eXpression (CALUX) in vitro cell bioassay is very promising. It allows the analyses of a high number of samples since it is relatively fast, inexpensive, and sensitive. However, this technique is not yet widely applied for screening or environmental monitoring. The main reasons are probably the lack of validation and the difficulty in interpreting the global biological response of the bioassay. In this paper, the strict quality control criteria set up for the validation of CALUX are described. The validation has shown good repeatability (relative standard deviation (RSD) = 9%) and good within-lab reproducibility (RSD = 15%) of the results. The quantification limit, in the conditions applied in this paper, is 1.25 pg CALUX-TEQ/g fat. Comparison of CALUX and GC-HRMS analysis was made for various marine matrixes (fishes, mussels, starfishes, sea birds, and marine mammals). Good correlations are usually observed, but there are systematic differences between the results. Attempts are made to identify the origin of the discrepancy between the two methods. |
IMIS is ontwikkeld en wordt gehost door het VLIZ.
