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Het OWA, het open archief van het Waterbouwkundig Laboratorium heeft tot doel alle vrij toegankelijke onderzoeksresultaten van dit instituut in digitale vorm aan te bieden. Op die manier wil het de zichtbaarheid, verspreiding en gebruik van deze onderzoeksresultaten, alsook de wetenschappelijke communicatie maximaal bevorderen.

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Characterization by 16S rRNA gene analysis and in situ hybridization of bacteria living in the hindgut of a deposit-feeding echinoid (Echinodermata) da Silva, S.G.; Gillan, D.C.; Dubilier, N.; De Ridder, C. (2006). Characterization by 16S rRNA gene analysis and in situ hybridization of bacteria living in the hindgut of a deposit-feeding echinoid (Echinodermata). J. Mar. Biol. Ass. U.K. 86(5): 1209-1213. dx.doi.org/10.1017/S0025315406014202 In: Journal of the Marine Biological Association of the United Kingdom. Cambridge University Press/Marine Biological Association of the United Kingdom: Cambridge. ISSN 0025-3154; e-ISSN 1469-7769, meer

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Beschikbaar in	Auteurs
VLIZ: Open Marine Archive 280365 [ download pdf ]

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Trefwoorden

Biodiversity Biology > Genetics Body parts > Digestive system > Digestive tract > Digestive system > Intestines > Digestive system > Hindgut Classification > Taxonomy > Chemotaxonomy Clones Isolating mechanisms > Genetic isolation Microflora Microorganisms > Bacteria Nucleic compounds > Glycosides > Nucleotides > Oligonucleotides Oligonucleotides Organisms > Microorganisms > Prokaryotes > Microorganisms > Bacteria > Filamentous bacteria Sediments > Chemical sediments > Nodules Techniques > Cytogenetic techniques > In situ hybridization > Fluorescence in situ hybridization Marien/Kust

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Auteurs		Top
da Silva, S.G., meer Gillan, D.C., meer Dubilier, N. De Ridder, C., meer

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Abstract

The hindgut caecum of the deposit-feeding echinoid Echinocardium cordatum harbours a symbiotic bacterial microflora, organized into layered mats around detrital particles owing to the proliferation of filamentous bacteria. The bacterial community was analysed using 16S rRNA gene analysis and fluorescence in situ hybridization. The purpose was to characterize its biodiversity and to identify its predominant members. The majority of the 16S sequences belong to the delta -Proteobacteria (61.5%), the Bacteroidetes and the Firmicutes constitute the two other main bacterial groups (respectively 23.1% and 15.4%). A total of 41% of the delta -Proteobacteria clones isolated belong to a bacterium of the genus Desulfonema for which a specific oligonucleotide probe was designed, enabling identification of its distribution in the nodules.

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