Over het archief
Het OWA, het open archief van het Waterbouwkundig Laboratorium heeft tot doel alle vrij toegankelijke onderzoeksresultaten van dit instituut in digitale vorm aan te bieden. Op die manier wil het de zichtbaarheid, verspreiding en gebruik van deze onderzoeksresultaten, alsook de wetenschappelijke communicatie maximaal bevorderen.
Dit archief wordt uitgebouwd en beheerd volgens de principes van de Open Access Movement, en het daaruit ontstane Open Archives Initiative.
Basisinformatie over ‘Open Access to scholarly information'.
Effects of cadmium, zinc and nitrogen status on non-protein thiols in the macroalgae enteromorpha spp. from the Scheldt estuary (SW Netherlands, Belgium) and Thermaikos Gulf (n Aegean Sea, Greece)
Malea, P.; Rijstenbil, J.W.; Haritonidis, S. (2006). Effects of cadmium, zinc and nitrogen status on non-protein thiols in the macroalgae enteromorpha spp. from the Scheldt estuary (SW Netherlands, Belgium) and Thermaikos Gulf (n Aegean Sea, Greece). Mar. Environ. Res. 62(1): 45-60. dx.doi.org/10.1016/j.marenvres.2006.03.002
In: Marine Environmental Research. Applied Science Publishers: Barking. ISSN 0141-1136; e-ISSN 1879-0291, meer
|  |
| Trefwoorden |
Algae
Chemical elements > Metals > Heavy metals > Cadmium
Chemical elements > Metals > Heavy metals > Zinc
Chemical elements > Nonmetals > Atmospheric gases > Nitrogen
Ulva Linnaeus, 1753 [WoRMS]
MED, Greece, Thermaikos Gulf [Marine Regions]
Brak water; Zoet water |
| Author keywords |
cadmium; Enteromorpha; glutathione; macroalgae; phytochelatins; zinc |
| Auteurs | | Top |
- Malea, P.
- Rijstenbil, J.W., meer
- Haritonidis, S.
|
|
|
| Abstract |
Enteromorpha prolifera (Scheldt Estuary) and E. linza (Thermaikos Gulf) were incubated at three salinities with 100 and 200 µg L-1Cd and Zn. The objective was to measure effects of Cd, Zn and nitrogen (N) status on the pools of metal-binding non-protein thiols: glutathione and phytochelatins, (?-glutamyl-cysteinyl)n-glycine (PC). In E. linza, ammonium pools were higher, but amino acid pools, total N and protein contents were lower than in E. prolifera. Reduced glutathione (GSH) pools were positively correlated with free glutamate and protein contents. In E. linza GSH pools increased and the ratio of reduced to oxidized glutathione (GSH:(GSH + 0.5GSSG)), an indicator of oxidative stress, decreased with Cd contents, indicating Cd-induced glutathione oxidation. Total glutathione pools (reduced plus oxidized) ranged from 16 nmol S gdwt-1 in controls (at 0.5 µmol Cd gdwt-1) to 179 nmol S gdwt-1 (at 1.9 µmol Cd gdwt-1) at the highest cadmium dosage. Cadmium stimulated PC synthesis in E. prolifera which suggests that in N-rich algae, glutathione pools were high enough for PC synthesis. In both species GSH and protein increased with Zn contents, whereas GSH:(GSH + 0.5GSSG) decreased, which would indicate Zn-induced oxidative stress; in E. linza, at the highest salinity the glutathione redox ratio decreased from 0.61 (at 2.9 µmol Zn gdwt-1) to 0.26 (at 4.9 nmol S gdwt-1) (at 0.5 mol Cd gdwt-1). PCs were not synthesized in response to Zn, which may have resulted in Zn-induced GSH oxidation. The presence of both oxidative effects (Cd, Zn) and detoxification (Cd) could be identified by observing the responses of glutathione and PC pools to metal stress. |
IMIS is ontwikkeld en wordt gehost door het VLIZ.
